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目的:测定先兆子痫患者母、脐血NK细胞增殖能力及杀伤功能,探讨先兆子痫的病因。方法:采用MTT法和51Cr释放法分别对先兆子痫患者(n=18)和正常晚孕妇女(n=18)的母血、脐血NK细胞进行细胞增殖能力和杀伤功能测定,同时测量NK细胞含量,并以NK-92细胞作为阳性对照。结果:先兆子痫患者和正常晚孕妇女脐血NK细胞数均高于母血(P<0.05);先兆子痫患者NK细胞增殖能力高于正常晚孕组(P<0.05);2组中脐血NK细胞增殖能力高于母血(P<0.05);先兆子痫患者NK细胞杀伤能力高于正常晚孕组(P<0.05)。结论:先兆子痫患者体内NK细胞数量及功能均表现增强状态,且脐血明显高于母血,从而在妊娠过程中影响母胎免疫耐受。
Objective: To determine the proliferation and cytotoxicity of NK cells in female and umbilical cord blood of patients with preeclampsia and to explore the etiology of preeclampsia. Methods: MTT assay and 51Cr release assay were used to measure the proliferation and cytotoxicity of maternal blood and umbilical cord blood NK cells in preeclampsia (n = 18) and normal pregnant women (n = 18) Cell content, and NK-92 cells as a positive control. Results: The number of NK cells in cord blood of preeclampsia and normal pregnant women were higher than that of maternal blood (P <0.05). The proliferation of NK cells in preeclampsia was higher than that in normal pregnant women (P <0.05) The proliferation of cord blood NK cells was higher than that of maternal blood (P <0.05). The NK cell killing capacity of patients with preeclampsia was higher than that of normal pregnancy group (P <0.05). CONCLUSION: The number and function of NK cells in patients with preeclampsia are enhanced, and the umbilical cord blood is significantly higher than that of maternal blood, thus affecting the immunological tolerance of the mother during pregnancy.