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目的 研究白介素 1受体相关激酶 2 (IRAK 2 )在白介素 1(IL 1)与肿瘤坏死因子 (TNF)激活核因子 κB(NF κB)过程中的作用。方法 IRAK 2反义寡核苷酸转染人胚肾细胞 (2 93细胞 ) ,阻断IRAK 2的表达 ,用IL 1及肿瘤坏死因子刺激细胞后 ,用夹心ELISA方法检测NF κB活性。结果 以IRAK 2反义寡核苷酸预处理可明显减弱IL 1诱导的NF κB活性 ,此效应强度存在时间和浓度依赖性 ,但I RAK 2反义寡核苷酸对肿瘤坏死因子诱导的NF κB活性无影响。结论 IRAK 2反义寡核苷酸对IL 1和TNF激活NF κB有不同影响。IRAK 2在IL 1诱导的NF κB信号转导通路中起关键作用
Objective To investigate the role of interleukin-1 receptor-associated kinase 2 (IRAK 2) in the activation of nuclear factor κB (NF-κB) by interleukin-1 (IL-1) and tumor necrosis factor (TNF). Methods IRAK 2 antisense oligonucleotides were transfected into human embryonic kidney cells (293 cells) to block the expression of IRAK 2. The cells were stimulated with IL - 1 and tumor necrosis factor - α (TNF - α). The activity of NF κB was detected by sandwich ELISA. Results Pretreatment with IRAK 2 antisense oligonucleotide significantly attenuated IL 1 -induced NF κB activity in a time and concentration-dependent manner. However, the effect of I RAK 2 antisense oligonucleotide on tumor necrosis factor-induced NF κB activity had no effect. Conclusion IRAK 2 antisense oligonucleotides have different effects on the activation of NFκB by IL-1 and TNF. IRAK 2 plays a key role in the IL-1-induced NF-κB signaling pathway