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目的:探讨肺炎衣原体感染高胆固醇饮食小鼠后血清TNF-α、IL-6、IL-10水平的变化及黄芩苷的治疗作用。方法:小鼠随机分为黄芩苷高剂量组、黄芩苷低剂量组、阿奇霉素组、高胆固醇+CPn感染组、正常对照组。喂饲高胆固醇饲料1周后,经一侧鼻腔吸入含CPn的培养液,每周1次,共3次。最后一次接种5天后开始给药至实验结束,第1次接种后第18周处死全部动物。结果:高胆固醇+CPn感染组血清TNF-α、IL-6、IL-10水平不同程度升高,三个治疗组三种炎症因子水平不同程度下降。结论:CPn感染可使高脂饮食C57BL/6J小鼠血清TNF-α、IL-6、IL-10水平升高,早期给予高、低剂量黄芩苷或阿奇霉素治疗可不同程度地调节这三种炎症因子的水平。
Objective: To investigate the changes of serum TNF-α, IL-6 and IL-10 levels in mice with chlamydia pneumoniae infection with high cholesterol diet and the therapeutic effects of baicalin. METHODS: Mice were randomly divided into high dose group of baicalin, low dose group of baicalin, azithromycin group, high cholesterol+CPn infection group, and normal control group. One week after feeding the high-cholesterol diet, CPn-containing medium was inhaled through one side of the nose once a week for a total of 3 times. The administration was started 5 days after the last inoculation until the end of the experiment, and all animals were sacrificed 18 weeks after the first inoculation. RESULTS: Serum TNF-α, IL-6, and IL-10 levels increased in the high cholesterol + CPn infection group, and the levels of the three inflammatory factors in the three treatment groups decreased to different degrees. Conclusion: CPn infection can increase the levels of serum TNF-α, IL-6, and IL-10 in high-fat diet C57BL/6J mice. Early and high-dose baicalin or azithromycin can regulate these three kinds of inflammation to varying degrees. The level of the factor.