论文部分内容阅读
目的:探讨齐墩果酸(OA)通过促进凋亡、抑制mTOR信号通路从而抑制骨肉瘤生长的分子机制。方法:18只裸鼠,成功构建骨肉瘤模型后,对照组(6只)给予正常饲养;实验Ⅰ(6只)、Ⅱ组(6只)给予OA每日灌胃。观察裸鼠骨肉瘤瘤体外观形态大小变化,测量瘤体体积变化,计算抑瘤率;取瘤体组织Western blot检测凋亡蛋白Caspase-3、PARP变化情况,检测mTOR信号通路中主要蛋白p-AKT、p-S6、p-4EBP1含量变化。结果:实验Ⅰ、Ⅱ组中瘤体组织体积及重量均明显较对照组小(P<0.01);肿瘤组织中凋亡蛋白Caspase-3、PARP裂解明显比对照组中增多;瘤体组织中mTOR信号通路中主要蛋白p-AKT、p-mTOR、p-S6、p-4EBP1含量明显较对照组中减少(P<0.05)。结论:OA在体内能明显抑制瘤体生长,通过促进肿瘤细胞凋亡、抑制mTOR信号通路起作用。
Objective: To investigate the molecular mechanism of oleanolic acid (OA) inhibiting the growth of osteosarcoma by promoting apoptosis and inhibiting the mTOR signaling pathway. Methods: 18 nude mice were successfully established osteosarcoma model, the control group (6) were given normal feeding; experimental Ⅰ (6), Ⅱ group (6) given intragastric administration of OA. The morphological changes of tumor in nude mice were observed. The change of tumor volume was measured and the tumor inhibition rate was calculated. The changes of apoptosis protein Caspase-3 and PARP in tumor tissue were detected by Western blot. The expression of major protein p- AKT, p-S6, p-4EBP1 content changes. Results: The volume and weight of tumor tissue in group Ⅰ and group Ⅱ were significantly lower than those in control group (P <0.01). The cleavage of apoptotic protein Caspase-3 and PARP in tumor tissue was more than that in control group. The expression of mTOR The levels of major proteins p-AKT, p-mTOR, p-S6 and p-4EBP1 in the signal pathway were significantly decreased compared with the control group (P <0.05). Conclusion: OA can obviously inhibit the growth of tumor in vivo, and promote the apoptosis of tumor cells and inhibit the mTOR signaling pathway.