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目的:探讨AFPmRNA在人肝细胞癌中的表现规律。方法:用RT-NestedPCR技术检测30例肝癌组织及癌旁组织AFPmRNA;放免法检测其血清AFP。结果:28例癌组织AFPmRNA阳性(28/30),2例癌旁组织AFPmRNA阳性;9例血清AFP阴性者,有7例组织AFPmRNA阳性;2例癌组织AFPmRNA及血清AFP均阴性。结论:人肝细胞癌AFP基因表达调控形式不同,AFPmRNA在早期诊断肝细胞癌中有一定价值。
Objective: To investigate the expression of AFP mRNA in human hepatocellular carcinoma. Methods: RT-Nested PCR was used to detect AFP mRNA in 30 cases of hepatocellular carcinoma and adjacent tissues. Serum AFP was detected by radioimmunoassay. Results: AFPmRNA was positive in 28 cases (28/30). Positive AFPmRNA was found in 2 cases of adjacent tissues. 9 cases were negative in serum AFP and 7 cases were positive for AFPmRNA. AFPmRNA and AFP were negative in 2 cases. Conclusion: The expression of AFP gene in human hepatocellular carcinoma is different. The AFPmRNA has a certain value in the early diagnosis of hepatocellular carcinoma.