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目的:采用不同方法诱导博莱霉素(BLM)致大鼠肺纤维化的模型,探讨符合人类肺纤维化病程的动物模型。方法:分别经气管、腹腔给予BLM致大鼠肺纤维化模型,于第1、14、28天处死,取肺组织制备病理切片,行HE染色后评价两种动物模型各时间段肺组织形态学变化,测量肺泡壁和胸膜厚度;测定肺组织匀浆中羟脯氨酸(HYP)含量,对两种模型进行评价。结果:(1)气管内、腹腔内给药均可建立肺纤维化动物模型,但时间长短不一;(2)气管内给药大鼠肺内病灶分布以末梢细支气管为主,早期的肺内炎症变化较重,而腹腔内给药大鼠病灶分布以胸膜下、肺组织内为主,炎症反应相对较轻;(3)腹腔内给药大鼠第28天肺组织匀浆HYP含量明显高于气管内给药(P<0.05)。结论:腹腔内给药建立的大鼠肺纤维化模型可能较近似于人类特发性肺纤维化的病程及病理改变。
OBJECTIVE: To investigate the pulmonary fibrosis model induced by bleomycin (BLM) in rats by different methods and to investigate the animal model of pulmonary fibrosis. Methods: The model of pulmonary fibrosis induced by BLM was induced by trachea and peritoneum. The rats were sacrificed on the 1st, 14th and 28th days. The lungs were taken for histopathological examination. The lung histomorphology of the two animal models was evaluated after HE staining. Changes in the alveolar wall and pleural thickness were measured. Hydroxyproline (HYP) content in lung homogenates was measured and the two models were evaluated. Results: (1) Animal models of pulmonary fibrosis could be established by intratracheal and intraperitoneal administration, but the time varied; (2) The distribution of intrapulmonary lesions in the lungs was mainly peripheral bronchioles. The early lung The inflammatory changes were relatively mild in the subpleural and lung tissues. (3) The content of HYP in the lung homogenate of the rats on the 28th day after intraperitoneal administration was significantly Higher than intratracheal administration (P <0.05). Conclusion: The rat model of pulmonary fibrosis established by intraperitoneal administration may be more similar to the course and pathological changes of human idiopathic pulmonary fibrosis.