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目的:探讨不同程度快动眼睡眠(REM)期睡眠剥夺(sleep deprivation,SD)及莫达非尼干预后大鼠下丘脑Orexin A神经元的表达。方法:将成年雄性Sprague-Dawley大鼠随机分为和SD组和对照组,SD组又分为用药组(drug group,DG)和非用药组(non-drug group,NDG),每组分SD12,24,48,72,96h共5个小组;对照组(cage control,CC)1个小组,正常饲养于笼中。每小组3只大鼠。采用小平台水环境法建立大鼠REM期SD模型。免疫组化方法观察大鼠下丘脑Orexin A阳性神经元的数量。结果:Orexin A阳性表达在SD12,24h时长的DG组与NDG组表达较CC组均有增加(P<0.05)而二者之间差别不明显(P>0.05);在SD48,72,96h时长的NDG组的表达较CC组下降(P<0.05),而DG组和CC组间无显著差异(P>0.05)且明显高于NDG组表达(P<0.05)。结论:推测莫达非尼可能是通过活化下丘脑促觉醒肽Orexin A的分泌和表达实现促觉醒作用。
Objective: To investigate the expression of Orexin A neurons in the hypothalamus of rats after rapid sleep (REM) sleep deprivation (SD) and modafinil intervention. Methods: Adult male Sprague-Dawley rats were randomly divided into SD group and control group. SD group was divided into drug group (DG) and non-drug group (NDG), SD12 , 24,48,72,96h total of five groups; control group (cage control, CC) a group of normal reared in the cage. 3 rats per group. Establishment of SD model of REM in SD rats by water platform method. The number of Orexin A positive neurons in the hypothalamus was observed by immunohistochemistry. Results: Compared with CC group, the expression of Orexin A in SD12,24h group was significantly higher than that in CC group (P <0.05), while the difference was not significant (P> 0.05); at SD48,72,96h (P <0.05), while there was no significant difference between DG group and CC group (P> 0.05), which was significantly higher than that of NDG group (P <0.05). CONCLUSION: It is speculated that modafinil may promote the awakening effect by activating the secretion and expression of Orexin A, a hypothalamic arousal peptide.