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目的:通过研究细胞因子和泌乳素(PRL)对球后成纤维细胞(RFS)表面细胞间粘附分子-1(ICAM-1)表达的影响,进一步了解甲状腺相关眼病(TAO)的发病机理,以探索新的防治途径.方法:将体外培养的正常人RFS与IFN-γ、IL-4、IFN-α和PRL孵育72H,利用直接免疫荧光技术和流式细胞仪检测其表面ICAM-1表达的情况.结果:正常人RFS中只有极少数细胞自发并较弱地表达ICAM-1,基础状态下的阳性百分数为(3.49±1.99),平均荧光强度为(44.84±20.59);而IFN-γ、IL-4、IFN-α能以剂量依赖方式显著增强ICAM-1的表达(P<0.05).PRL不仅可以直接刺激RFS表面ICAM-1的表达(P<0.05),其剂量相关曲线呈双相型,还能拮抗或协同IFN-γ(100U/ML)、IFN-α(1000U/ML)或IL-4(2.5NG/ML)的诱导作用.结论:IFN-γ、IL-4、IF-α和PRL对正常人RFS表面ICAM-1的表达有明显的调节作用,它们可能是启动或加重TAO自身免疫反应的重要因素.“,”Objective: To further understand the pathogenesis of thyroid associated ophthalmopathy and develop new curative ways, we studied the influences of cytokines and prolactin (PRL) on ICAM-1 expression on the surfaces of retroocular fibroblasts (RFs). Methods: With direct immunofluorescence technique and flowcytometry, we determined ICAM-1 expression on the surfaces of cultured normal human RFs incubated with IFN γ,IL 4,IFN α and(or) PRL for 72 hours. Results: Very few RFs spontaneously and weakly expresse...