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目的探讨表皮葡萄球菌与人脐静脉血管内皮细胞ECV304的黏附作用及其机制。方法将表皮葡萄球菌临床分离株通过刚果红琼脂检测胞间多糖黏附素(PIA);半定量生物被膜形成试验检测生物被膜表型;并在体外与ECV304细胞共同作用,在倒置显微镜下观察表皮葡萄球菌对ECV304细胞的黏附数。结果表皮葡萄球菌黏附ECV304细胞的数量随作用时间的延长而增加;PIA+/生物被膜表型+菌株与ECV304细胞的黏附数多于PIA+/生物被膜表型-菌株和PIA-/生物被膜表型-菌株,且差异有统计学意义,而PIA+/生物被膜表型-菌株和PIA-/生物被膜表型-菌株与细胞的黏附数差异无统计学意义。结论表皮葡萄球菌与血管内皮细胞的黏附有时间依赖性,能形成生物被膜的表皮葡萄球菌更易与血管内皮细胞黏附。
Objective To investigate the adhesion between Staphylococcus epidermidis and human umbilical vein endothelial cells (ECV304) and its mechanism. Methods Staphylococcus epidermidis clinical isolates were detected by Congo red agar intercellular adhesion polysaccharide (PIA); biofilm formation assay semi-quantitative biofilm phenotype; ECV304 cells in vitro and in vitro, under an inverted microscope to observe the epidermal grapes Cocci adhesion to ECV304 cells. Results The number of ECV304 cells adhered to Staphylococcus epidermidis increased with the prolongation of time. The number of PIA + / biofilm + strain and ECV304 cells adhered more than that of PIA + / biofilm - strain and PIA- / biofilm - However, there was no significant difference in the number of cells adhered to PIA + / biofilm-strain and PIA- / biofilm phenotype-strain. Conclusion Staphylococcus epidermidis and vascular endothelial cell adhesion in a time-dependent manner, can form a biofilm Staphylococcus epidermidis more easily with vascular endothelial cell adhesion.