论文部分内容阅读
目的:探究健康人成功接种乙肝疫苗后外周血单个核细胞(PBMCs)增殖及其Tbet、GATA-3、Foxp3、RORγt和Bcl-6五种CD4+T淋巴细胞亚群特异性转录因子的表达特征,评价细胞免疫应答状态。方法:本研究共采集64份外周血样本,分别来自43例疫苗免疫[HBs Ab(+)]和21例未疫苗免疫[HBs Ab(-)]的健康人。其中,有部分样本使用HBV重组核心抗原(r HBc Ag)进行诱导培养。通过CCK-8法检测细胞增殖情况;采用荧光定量PCR(FQ-PCR)法检测五种转录因子的m RNA表达水平。结果:HBs Ab(+)组五种转录因子基础表达水平均高于HBs Ab(-)组,其中GATA-3、Foxp3和R/F比值有显著差异(P<0.001)。经r HBc Ag诱导后,HBs Ab(-)组五种转录因子的m RNA表达水平较基础水平均有意义地增加(P<0.05);而在HBs Ab(+)组中,则均有不同程度降低,其中GATA-3显著降低(P=0.044);且诱导后HBs Ab(-)组五种转录因子表达水平均高于HBs Ab(+)组(P<0.05)。结论:乙肝疫苗免疫成功人群的细胞免疫在基础和体外再次应答水平上与非疫苗免疫健康者均存在明显的差别。
AIM: To investigate the expression of peripheral blood mononuclear cells (PBMCs) proliferation and the expression of transcription factors specific to Tbet, GATA-3, Foxp3, RORγt and Bcl-6 CD4 + T lymphocyte subsets in healthy volunteers after hepatitis B vaccination , Evaluate the cellular immune response status. Methods: A total of 64 peripheral blood samples were collected from 43 healthy individuals who were immunized with the vaccine [HBs Ab (+)] and 21 without the vaccine [HBs Ab (-)]. Among them, some samples were induced by HBV recombinant core antigen (r HBc Ag). Cell proliferation was detected by CCK-8 assay. The m RNA expression levels of five transcription factors were detected by fluorescence quantitative PCR (FQ-PCR). Results: The basal expression levels of five transcription factors in HBs Ab (+) group were higher than that in HBs Ab (-) group, and the ratios of GATA-3, Foxp3 and R / F were significantly different (P <0.001). The expression of m RNA of five transcription factors of HBs Ab (-) group increased significantly (P <0.05) compared with the basal level after induced by r HBc Ag, but different in HBs Ab (+) group (P = 0.044). The expression levels of five transcription factors in HBs Ab (-) group were higher than that in HBs Ab (+) group (P <0.05). Conclusion: There is a significant difference in cellular immunity between successful hepatitis B vaccine immunization population and those with non-vaccine immune response both at basic and in vitro response level.