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为了评估蟾酥水提取物的体外抗炎作用,研究用LPS诱导RAW 264.7细胞和小鼠腹腔巨噬细胞建立细胞炎症模型,采用Griess法测定蟾酥水提取物对细胞上清液中NO水平的影响,采用ELISA法测定TNF-α、IL-1β和IL-6含量,采用荧光法测定细胞内ROS水平。结果表明:蟾酥水提取物对LPS诱导的RAW 264.7细胞和小鼠腹腔巨噬细胞所分泌的炎症因子NO、TNF-α、IL-1β和IL-6有明显的抑制作用,与模型组相比明显降低,同时蟾酥水提取物能够极显著降低LPS诱导的细胞内ROS水平(P<0.01)。说明蟾酥水提取物可能通过抑制炎症因子、降低细胞内活性氧水平减轻细胞的炎症反应,表现良好的抗炎作用。
In order to evaluate the in vitro anti-inflammatory effect of water extract of Toadstool, RAW 264.7 cells and mouse peritoneal macrophages were induced by LPS to establish the model of cellular inflammation. The effects of water extract of Toadstool on the level of NO in cell supernatant were determined by Griess method. The levels of TNF-α, IL-1β and IL-6 were measured by ELISA. The levels of intracellular ROS were measured by fluorescence method. The results showed that water extracts of Toadstool could significantly inhibit the production of inflammatory factors NO, TNF-α, IL-1β and IL-6 secreted by LPS-induced RAW 264.7 cells and peritoneal macrophages in mice, compared with the model group (P <0.01). At the same time, the water extracts of Toadstool could significantly reduce the intracellular ROS level induced by LPS (P <0.01). It showed that the water extract of Toadstool could exert a good anti-inflammatory effect by inhibiting the inflammatory cytokines and reducing the level of reactive oxygen species in cells.