论文部分内容阅读
目的 :探讨T辅助 (Th)和T毒性 (Tc)淋巴细胞亚群紊乱与原发性肾病综合征患儿疾病发生的关系。方法 :运用三色荧光标记法流式细胞术检测 15例复发的原发性肾病患儿外周血Th和Tc细胞亚群百分率 (% ) ,并以 16例缓解期肾病患儿和 10例正常儿童作对照。 结果 :正常儿童外周血Th1、Th2和Th0或Tc1、Tc2和Tc0细胞百分率分别为 (13 4± 4 36 ) % ,(2 5 3± 1 97) %和 (1 2 5± 0 92 ) %或 (2 6 2± 9 5 7) % ,(1 14± 0 98) %和 (0 42±0 45 ) %。复发的肾病患儿Th1、Th0或Tc1细胞百分率均减低 ,分别为 (1 2 0± 0 91) % (P <0 0 1)、(0 40± 0 39) %(P <0 0 5 )或 (8 6 2± 7 5 2 ) % (P <0 0 5 ) ,Th2细胞百分率增高 ,为 (7 6 5± 4 5 3) % (P <0 0 1) ,而Tc2和Tc0细胞百分率无变化 ,分别为 (1 5 2± 2 0 7) %和 (0 30± 0 31) % (P >0 0 5 )。缓解期肾病患儿Th和Tc细胞亚群百分率均正常 ,Th1、Th2、Th0细胞分别为 (12 0± 4 75 ) % ,(2 87± 2 46 ) %和 (1 31± 0 87) % ;Tc1、Tc2、Tc0细胞分别为 (2 1 6±7 16 ) % ,(0 95± 0 97) %和 (0 37± 0 36 ) % (P >0 0 5 )。Th1/Th2和Tc1/Tc2比值在正常儿童分别为 5 41± 2 77和79 3± 81 5 ,在复发的患儿均明显下降 ,分别为 0 77?
Objective: To investigate the relationship between the disorder of T helper (T) and T toxicity (Tc) lymphocyte subsets and the incidence of nephrotic syndrome in children. Methods: The percentage of Th and Tc subsets in peripheral blood of 15 children with recurrent primary nephropathy was detected by three-color fluorescence-labeled flow cytometry and 16 children with remission nephropathy and 10 normal children As a control. Results: The percentages of Th1, Th2 and Th0 or Tc1, Tc2 and Tc0 in normal children were (13 4 ± 4 36)%, (2 5 3 ± 1 97)% and (1 2 5 ± 0 92)% (2 6 2 ± 9 5 7)%, (1 14 ± 0 98)% and (0 42 ± 0 45)%, respectively. The percentages of Th1, Th0 or Tc1 in children with recurrent nephropathy were all decreased (100 ± 0 91)%, 0 40 ± 0 39% (P 0 05) or (8 6 2 ± 7 5 2)% (P 0 05), and the percentage of Th2 cells was (755 453)% (P 0 01), while the percentage of Tc 2 and Tc 0 cells did not change (15 2 ± 2 0 7)% and (0 30 ± 0 31)%, respectively (P 0 05). The percentages of Th and Tc subsets in children with remission nephropathy were all normal, and those of Th1, Th2 and Th0 were (12 0 ± 4 75)%, (2 87 ± 2 46)% and (1 31 ± 0 87)%, respectively. The Tc1, Tc2 and Tc0 cells were (21 6 ± 7 16)%, (0 95 ± 0 97)% and (0 37 ± 0 36)%, respectively (P 0 05). The ratio of Th1 / Th2 to Tc1 / Tc2 in normal children were 5 41 ± 2 77 and 79 3 ± 81 5, respectively, but decreased significantly in children with relapse (0 77%