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目的 研究帕金森病 (PD)动物模型黑质的超微结构及灵芝孢子粉对这些超微结构的影响。方法 30只健康雄性SD大鼠被分成 3组 ,每组 10只 ,其中PD组采用立体定向仪注入微量 6 羟多巴 (6 OHDA)至右侧中脑黑质 ,诱发PD模型。将成功的PD模型鼠用电镜固定液灌流固定脑组织 ,然后电镜观察黑质超微结构。灵芝孢子粉组先用灵芝孢子粉预处理 3天 ,再用立体定向仪注入微量 6 OHDA至右侧中脑黑质 ,并连续应用灵芝孢子粉直至4周 ,选择旋转次数最多的 6只大鼠电镜观察黑质超微结构。正常对照组黑质注入 0 2 %抗坏血酸生理盐水混合液。结果 PD组大鼠有较多的凋亡的神经细胞 ,线粒体嵴不清、内质网扩张 ,而正常对照组没有这些变化。灵芝孢子粉预处理组存在轻度的线粒体嵴不清、内质网扩张 ,偶见凋亡的神经细胞。结论 从形态学上说明神经细胞的凋亡在PD的发病机制中具有重要作用 ,同时灵芝孢子粉对黑质神经细胞具有保护作用。
Objective To study the ultrastructure of substantia nigra in Parkinson’s disease (PD) animal model and the effects of Ganoderma lucidum spores on these ultrastructures. Methods Thirty healthy male Sprague-Dawley rats were divided into three groups of 10 rats. PD group was injected PD with 6-OHDA into substantia nigra of right middle cerebral artery by stereotactic instrument in PD group. The successful PD model rats were fixed by electron microscopy fixed brain tissue, and then electron microscopy of the substantia nigra ultrastructure. Ganoderma lucidum spore powder group with Ganoderma lucidum spore powder pretreatment for 3 days, and then stereotactic instrument into a small amount of 6 OHDA to the right side of the brain substantia nigra, and continuous application of Ganoderma lucidum spore powder until 4 weeks, select the most rotating 6 rats Electron microscopy of the substantia nigra ultrastructure. The normal control group was injected with 0.2% ascorbate saline solution. Results There were more apoptotic nerve cells in PD group, unclear mitochondrial cristae and endoplasmic reticulum, but no changes in normal control group. Ganoderma lucidum spore powder pretreatment group there is mild mitochondrial crista, endoplasmic reticulum dilatation, occasionally apoptotic nerve cells. Conclusion Morphology shows that apoptosis of neural cells plays an important role in the pathogenesis of PD. At the same time, Ganoderma lucidum spore powder has a protective effect on the substantia nigra neuronal cells.