2014年,天津口岸从日本进境盆景中截获3种短体线虫和1种根结线虫。通过形态学和DNA条形码方法将截获的短体线虫鉴定为朱顶红短体线虫(Pratylenchus hippeastri)、伤残短体线虫(P.vulnus)和穿刺短体线虫(P.penetrans)。根据形态学并结合特异PCR和DNA条形码方法,将截获的根结线虫2龄幼虫鉴定为南方根结线虫(Meloidogyne incognita)。朱顶红短体线虫是短体线虫属非中国种,为我国进境植物检疫性有害生物,在挪威槭和榉树盆景中截获该线虫为国内外首次报道。 In 2014, three species of nematode and one root-knot nematode were intercepted in Tianjin Port from Japanese bonsai. Intercepted short-body nematodes were identified as Pratylenchus hippeastri, P. vulnus and P. penetrans by morphological and DNA barcoding methods. The second-instar larvae of M. incognita were identified as Meloidogyne incognita according to morphology and combined with specific PCR and DNA barcoding methods. Hippeastrum nematode is a non-Chinese species of Nematoda. It is the first quarantine pest in China that the nematode was intercepted in Norway maple and beech bonsai.