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目的探讨乙型肝炎病毒(HBV)感染孕妇的外周血单个核细胞(PBMC)在宫内感染中的作用。方法选择血清HBV DNA阴性且PBMC HBV DNA阳性孕妇及其新生儿30对作为实验组,HBV标志物均为阴性孕妇及其新生儿10对作为对照组。采用巢式聚合酶链反应(PCR)法检测血清及PBMC HBV DNA,半巢式PCR检测新生儿PBMC中来自母亲的细胞。结果实验组30例新生儿中,血清HBV DNA 阳性4例,PBMC HBV DNA阳性8例,均为弱阳性。8例PBMC HBV DNA阳性新生儿中,4例人类谷胱甘肽S转移酶(GST)M1基因阳性,其中5例母亲的GSTM1基因阳性;3例新生儿GSTM1阴性,母亲GSTM1 阳性,在这3例新生儿PBMC中均检测到GSTM1基因。对照组10例新生儿血清和PBMC HBV DNA均阴性。结论 HBV感染新生儿的部分外周血单个核细胞可能来自母体,并作为HBV宫内感染的媒介。
Objective To investigate the role of peripheral blood mononuclear cells (PBMCs) in intrauterine infection in pregnant women with Hepatitis B virus (HBV) infection. Methods Thirty pairs of serum HBV DNA negative and PBMC HBV DNA positive pregnant women and their newborns were selected as the experimental group. All the HBV markers were negative and 10 newborns were used as the control group. Serum and PBMC HBV DNA were detected by nested polymerase chain reaction (PCR) and semi-nested PCR was used to detect the mothers’ cells in neonates PBMC. Results Among the 30 newborns in the experimental group, 4 were positive for serum HBV DNA and 8 were positive for HBV DNA in PBMC, all of which were weakly positive. Of the 8 PBMC HBV DNA-positive newborns, 4 were human glutathione S transferase (GST) M1 gene positive, of which 5 were mothers with GSTM1 gene positive; 3 were neonatal GSTM1 negative and their mothers were GSTM1 positive, GSTM1 gene was detected in all newborn PBMCs. The control group of 10 neonates serum and PBMC HBV DNA were negative. Conclusion Some peripheral blood mononuclear cells from newborns infected with HBV may come from the mother and serve as a mediator of HBV intrauterine infection.