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为阐明肾综合征出血热（ＨＦＲＳ）发病机制，用免疫组化法检查了３０例患者ＨＦＲＳ病毒的膜蛋白（ＭＰ）、核蛋白（ＮＰ）在外周血单个核细胞（ＰＢＭＣ）中的表达情况，同时用放免等技术检测了血浆内皮素（ＥＴ）、Ｐ物质（ＳＰ）和肾功能相关指标。发现ＨＦＲＳ患者从４～５病日起至１３病日，ＰＢＭＣ中均有ＭＰ和ＮＰ表达，但随着病情的好转，其表达强度逐渐减弱。经染色鉴定ＭＰ与ＮＰ阳性细胞主要是单核细胞。入院时ＰＢＭＣ中ＭＰ和ＮＰ表达强度并不相同，ＭＰ的表达强度与血浆ＥＴ／ＳＰ比值、病情及肾脏损伤轻重呈平行关系。提示ＨＦＲＳ病毒能侵犯ＰＢＭＣ并在其中复制，表达病毒结构蛋白，而ＭＰ与ＨＦＲＳ病毒的致病作用相关；病程中血浆ＥＴ／ＳＰ比值升高是使肾损加重的重要因素。
To clarify the pathogenesis of hemorrhagic fever with renal syndrome (HFRS), the expression of membrane protein (MP) and nuclear protein (NP) in peripheral blood mononuclear cells (PBMCs) of 30 HFRS patients was examined by immunohistochemistry , While using radioimmunoassay and other techniques to detect plasma endothelin (ET), substance P (SP) and renal function related indicators. Found that HFRS patients from 4 to 5 days from the date of disease to 13 days, PBMC both MP and NP expression, but as the disease improves, its expression intensity gradually weakened. After staining identified MP and NP positive cells are mainly monocytes. The expression intensity of MP and NP in PBMC was not the same on admission, and the expression intensity of MP was in parallel with the ET / SP ratio of plasma and the severity of illness and kidney injury. It is suggested that HFRS virus can infect and replicate PBMC and express viral structural protein, while MP is associated with pathogenicity of HFRS virus. Increasing plasma ET / SP ratio during the course of the disease is an important factor that aggravates renal damage.