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目的 探讨碱性成纤维细胞生长因子 (b FGF)对人α1 ( )胶原基因启动子活性的影响 ,以及与转化生长因子 - β1 (TGF- β1 )之间的相互作用 ,为防治增生性瘢痕提供依据。 方法 正常皮肤及瘢痕成纤维细胞原代、传代培养。采用 Fu GENE转染试剂 ,分别瞬间转染含人α1 ( )胶原基因 5’端序列 - 2 .5 kb与报告基因氯霉素乙酰基转移酶(CAT)的重组体 ph COL2 .5至正常皮肤及瘢痕成纤维细胞。 ELISA法测定 b FGF及 TGF- β1 作用 2 4小时后 ,转染ph COL2 .5的两种成纤维细胞的报告基因 CAT表达量。 结果 b FGF能抑制转染 ph COL2 .5重组体的正常皮肤及瘢痕成纤维细胞 CAT表达量 ,且能拮抗 TGF-β1 对转染 ph COL2 .5重组体的两种成纤维细胞 CAT表达的上调作用。与对照组相比有统计学意义 (P<0 .0 5)。 结论 正常皮肤及瘢痕成纤维细胞中 ,b FGF均能抑制人 α1 ( )胶原基因的启动转录 ,且能拮抗 TGF-β1 对人α1 ( )胶原基因启动活性的上调作用 ,b FGF抗纤维机制有望为增生性瘢痕的防治提供新思路
Objective To investigate the effect of basic fibroblast growth factor (b FGF) on the promoter activity of human α1 (superscript) collagen gene and its interaction with transforming growth factor - β1 (TGF - β1), and provide a basis for prevention and treatment of hypertrophic scars in accordance with. Methods Normal skin and scar fibroblasts primary, subculture. Fu GENE transfection reagent was used to transiently transfect the recombinant human body phorboltransferase (CAT) containing the 5 ’end of the human α1 (-) collagen gene 2.55 kb and the reporter gene chloramphenicol acetyltransferase (CAT) into the normal skin And scar fibroblasts. The expression of CAT in two fibroblasts transfected with ph COL2.5 was measured by ELISA after bFGF and TGF-β1 were treated for 24 hours. Results b FGF inhibited the expression of CAT in normal skin and scar fibroblasts transfected with ph COL2.5 recombinant and inhibited the up-regulation of CAT expression in both fibroblasts transfected with ph COL2.5 recombinant effect. Compared with the control group, there was statistical significance (P <0.05). CONCLUSION: Both bFGF and normal fibroblasts can inhibit the transcriptional activation of human α1 (collagen) gene and upregulate the promoter activity of human α1 (superscript) collagen by TGF-β1. The anti-fibrin mechanism of bFGF is expected to be promising Provide a new idea for the prevention and treatment of hypertrophic scars