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目的:探讨NDRG1对体外培养的人肠癌细胞系失巣凋亡的影响。方法:采用慢病毒系统将NDRG1表达单元转入人肠癌细胞系SW620、HCT8中,建立相应的过表达稳定细胞系;通过siRNA的方法干扰HCT116和LOVO细胞系中NDRG1的表达,分别在非贴壁培养的情况下培养48小时,采用流式细胞术和TUNEL染色检测细胞的凋亡情况。结果:在贴壁培养条件下,NDRG1过表达并没有显著影响肠癌细胞的生长及增殖,而NDRG1特异性siRNA干扰HCT116细胞中NDRG1的表达后,其凋亡率无明显变化(P>0.05)。在悬浮培养条件下,NDRG1过表达的肠癌细胞的失巢凋亡率显著低于正常对照组(P<0.05),而用三种不同的siRNA干扰HCT116及LOVO细胞中NDRG1的表达后,其失巢凋亡率均显著高于正常对照组(P<0.05)。结论:NDRG1在体外可抑制人肠癌细胞的失巢凋亡。
Objective: To investigate the effect of NDRG1 on apoptosis and apoptosis of human colorectal cancer cell lines in vitro. Methods: NDRG1 was transfected into human colorectal cancer cell lines SW620 and HCT8 by lentivirus system, and the corresponding overexpression stable cell lines were established. The expression of NDRG1 in HCT116 and LOVO cell lines was disturbed by siRNA, Cultured for 48 hours in the presence of a wall, and the apoptosis of the cells was examined by flow cytometry and TUNEL staining. Results: NDRG1 overexpression did not significantly affect the growth and proliferation of colorectal cancer cells in adherent culture. NDRG1-specific siRNA did not affect the apoptosis rate of NDRG1 in HCT116 cells (P> 0.05) . In suspension culture, the rate of anoikis in NDRG1 overexpressing colon cancer cells was significantly lower than that in the normal control group (P <0.05). When three different siRNAs were used to interfere the expression of NDRG1 in HCT116 and LOVO cells, The rate of anoikis was significantly higher than that of the normal control (P <0.05). Conclusion: NDRG1 can inhibit anoikis in human colorectal cancer cells in vitro.