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目的研究依达拉奉对小鼠缺血性脑损伤的保护作用。方法昆明小鼠36只随机分为假手术组、单纯缺血组(单缺组)、术后即刻给药组(即刻组)、术后6h给药组(6h组)、术后12h给药组(12h组)、术后24h给药组(24h组),并建立小鼠单侧永久性大脑中动脉栓塞模型,依达拉奉腹腔注射,术后每天对小鼠进行神经功能缺损评分,共3d;脑组织切片用TTC(红四氯唑)染色后测量并分析脑梗死体积占总体积近似百分比。结果单缺组与即刻组、6h、12h组比较有显著性差异,后者与24h组比较亦有显著性差异。脑梗死体积占总体积近似百分比和神经功能缺损评分。结论单侧永久性大脑中动脉栓塞后12h内使用依达拉奉可以减少脑梗死体积,改善神经功能。
Objective To study the protective effect of edaravone on ischemic brain injury in mice. Methods Thirty-six Kunming mice were randomly divided into sham operation group, ischemia group (single defect group), postoperative immediate administration group (immediate effect group), postoperative 6h administration group (6h group) (12h group) and 24h postoperatively (24h group). The model of unilateral middle cerebral artery occlusion (MCAO) was established in mice and intradermal injection of edaravone. The neurological deficit score of mice was evaluated daily, A total of 3d; brain tissue sections were stained with TTC (red tetrazolium) to measure and analyze the approximate volume of the total volume of cerebral infarction percentage. Results There was a significant difference between single-deficient group and immediate group, 6h, 12h group, while there was significant difference between the latter group and 24h group. The percentage of infarct volume in the total volume and the neurological deficit score. Conclusion The use of edaravone within 12 hours after unilateral permanent middle cerebral artery occlusion can reduce the volume of cerebral infarction and improve neurological function.