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采用我国当前流行的7个小麦条锈菌(菌系),在高温条件下对其进行成株期抗条锈性鉴定,并用条锈菌CYR32对小麦品种小偃54与铭贤169的杂交后代及其双亲进行高温成株期抗条锈性遗传分析,以揭示小偃54成株期抗条锈性遗传机制。结果显示,小偃54成株期对多个条锈菌小种具有良好的抗病性,对CYR32的抗病性由2对隐性核基因独立控制,分别暂命名为Yrxy54-1和Yrxy54-2,并对Yrxy54-1进行分子标记定位。从528个RGAP引物组合中筛选到4个与抗病基因Yrxy54-1紧密连锁的多态性标记M1、M2、M3和M4,分布于Yrxy54-1的两侧,遗传距离分别为15.8、16.6、7.3和9.97cM。遗传分析结合分子标记结果表明,Yrxy54-1是一个与已知抗条锈基因不同的新基因。
Seven strains of wheat stripe rust (bacterial strain) currently prevailing in China were used to identify their resistance to stripe rust at the adult stage under high temperature. The stripe rust CYR32 was used to identify the cross between offspring of wheat varieties Xiaoyan 54 and Mingxian 169 And their parents to carry out genetic analysis of the resistance to stripe rust in adult plants at high temperature in order to reveal the genetic mechanism of resistance to stripe rust in adult plants of Xiaoyan 54. The results showed that the adult plants of Xiaoyan 54 had good disease resistance to several stripe rust races. The resistance to CYR32 was independently controlled by two pairs of recessive nuclear genes, which were named Yrxy54-1 and Yrxy54- 2, and Yrxy54-1 molecular marker location. Four polymorphic markers M1, M2, M3 and M4 closely linked to the resistance gene Yrxy54-1 were screened from 528 RGAP primer combinations and distributed on both sides of Yrxy54-1 with genetic distances of 15.8 and 16.6, 7.3 and 9.97cM. Genetic analysis combined with molecular marker results showed that Yrxy54-1 is a new gene different from the known stripe rust gene.