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目的:通过卵清蛋白注射液制作气道重构哮喘豚鼠模型,观察气道转化生长因子β1蛋白表达及电针对其的影响。方法:实验于2005-08/10在广州中医药大学实验动物中心完成。选用健康豚鼠48只,雌雄各半。将动物按随机数字表法分为正常对照组、电针治疗组、地塞米松组和模型组4组,每组12只。电针治疗组,地塞米松组和模型组动物于实验第1天腹腔注射100g/L卵清蛋白注射液1mL以致敏,正常对照组动物腹腔注射0.9%生理盐水1mL。第15天起作部动物隔天用卵蛋白粉末加上生理盐水配成10g/L卵蛋白溶液超声雾化吸入约20s。电针治疗组每次卵白蛋白喷雾攻击前0.5h开始电针治疗。地塞米松组于每次卵白蛋白喷雾攻击前0.5h腹腔注射100g/L地塞米松1mL。正常对照组和模型组不进行干预。治疗结束后,采用免疫组织化学法检测与气道重构相关的细胞因子转化生长因子β1在各组动物支气管和肺组织中的蛋白表达。结果:纳入的48只豚鼠,4只于造模过程中死亡而脱落,其余44只进入结果分析。各组豚鼠的支气管、肺组织切片中均见棕黄色颗粒状的免疫阳性反应,主要集中在气道上皮细胞、基底膜、肺间质、软骨和炎症细胞中。模型组转化生长因子β1阳性细胞数明显高于对照组,差异有显著性意义(P<0.01);电针治疗组与地塞米松组的支气管肺组织转化生长因子β1阳性细胞数低于模型组,差异有显著性意义(P<0.05)。结论:①哮喘豚鼠气道重构模型气道转化生长因子β1的蛋白表达增加,电针豚鼠背六穴能降低气道重构豚鼠模型支气管肺组织中的转化生长因子β1蛋白表达。②电针豚鼠背六穴可通过下调转化生长因子β1蛋白表达干预哮喘豚鼠的气道重构。
OBJECTIVE: To establish airway remodeled guinea pig model by ovalbumin injection and observe the effect of electroacupuncture on the expression of transforming growth factor-β1 in airway. Methods: The experiment was performed at Experimental Animal Center of Guangzhou University of Chinese Medicine from August to August 2005. Selection of healthy guinea pig 48, male and female half. The animals were divided into four groups according to random number table: normal control group, electroacupuncture treatment group, dexamethasone group and model group, with 12 rats in each group. The electroacupuncture treatment group, dexamethasone group and model group animals were sensitized by intraperitoneal injection of 1 mL of 100g / L ovalbumin injection on the first day of experiment, while normal control animals were injected with 1 mL of 0.9% physiological saline by intraperitoneal injection. The first 15 days for the Department of animal every other day with egg protein powder plus normal saline dubbed 10g / L ovalbumin solution ultrasonic atomization inhalation about 20s. Electroacupuncture treatment was started at 0.5h before electroosmotic treatment in each group. The dexamethasone group was intraperitoneally injected with 1 mL of 100 g / L dexamethasone 0.5 h prior to each ovalbumin challenge. Normal control group and model group did not intervene. After treatment, immunohistochemistry was used to detect the expression of TGF-β1 in airway remodeling in the bronchi and lung tissues of each group. Results: 48 guinea pigs were enrolled, 4 died during the modeling and shedding, and the rest 44 were involved in the result analysis. Bronchial and lung tissue sections of guinea pigs in each group showed brown granular immunopositive reaction, mainly in airway epithelial cells, basement membrane, interstitial lung, cartilage and inflammatory cells. The number of TGF-β1-positive cells in the model group was significantly higher than that in the control group (P <0.01). The number of TGF-β1-positive cells in the bronchial lung tissue of EA group and dexamethasone group was lower than that of the model group , The difference was significant (P <0.05). Conclusion: ①The expression of TGF-β1 in the airway remodeling model of asthmatic guinea pigs is increased, while the expression of TGF-β1 protein in the bronchopulmonary tissues of the airway remodeling guinea pig model is reduced by the electro-acupuncture at the dorsal point. ② Electro-acupuncture guinea pigs in the dorsal points can interfere the airway remodeling in asthmatic guinea pigs by down-regulating the expression of transforming growth factor-β1.