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本实验用豚鼠回肠，小鼠输精管和兔输精管为实验对象，用特异性阿片受体激动剂和拮抗剂为工具药，从定性，定量两方面研究了促甲状腺素释放激素（ＴＲＨ）与μ，δ，κ亚型阿片受体的关系。结果显示ＴＲＨ（０．０１ｍｍｏｌ／Ｌ）可拮抗Ｄ－丙２，Ｄ－亮５－脑啡肽（ＤＡＤＬＥ）抑制小鼠输精管的收缩反应，同时可使ＤＡＤＬＥ的对数浓度反应曲线（ＬＣＣＲＣ）右移，其拮抗参数ｐＡ２值为９．３，但ＴＲＨ不拮抗双氢埃托啡（ＤＨＥ）抑制豚鼠回肠及埃托啡（Ｅｔｏ）抑制兔输精管的收缩反应，对ＤＨＥ及Ｅｔｏ的ＬＣＣＲＣ无明显影响，结果提示ＴＲＨ拮抗阿片肽的作用有一定的受体亚型选择性，这种选择性可能与δ阿片受体有关
In this experiment, guinea pig ileum, mouse vas deferens and rabbit vas deferens were used as experimental subjects, and specific opioid receptor agonists and antagonists were used as a tool to study the effects of thyrotropin-releasing hormone (TRH) and μ, δ, κ subtypes opioid receptor relationship. The results showed that TRH (0.01mmol / L) could antagonize the D-leupid and D-enkephalin (DADLE) to inhibit vasoconstriction in mice and at the same time, the logarithmic concentration response curve (LCCRC) And its antagonistic parameter pA2 was 9.3. However, TRH did not antagonize DHE inhibiting the contraction of rabbit vas deferens in guinea pig ileum and etode and had no significant effect on the LCCRC of DHE and Eto , The results suggest that TRH antagonism of opioid peptide receptor has a certain subtype selectivity, this selectivity may be related to δ opioid receptor