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目的 :研究阻断泛素 -蛋白酶体通路对食管癌细胞生长及端粒酶活性的影响。方法 :将不同浓度的MG - 132加入食管癌细胞株Eca970 6 ,特异性阻断其泛素 -蛋白酶体通路 ,用四甲基偶氮唑蓝 (MTT)法测定细胞生长抑制效应 ,显微镜下观察细胞形态变化 ,流式细胞仪 (FCM)检测细胞周期及凋亡 ,DNA片段分析进一步证实凋亡的存在 ,并对端粒酶的活性进行检测。结果 :MG - 132对食管癌细胞株Eca970 6有显著的生长抑制作用 ,且呈现剂量与时间的依赖性 ,显微镜下可见明显的细胞病变 ,细胞变圆、变小、脱落 ,FCM显示MG - 132作用于食管癌细胞后 ,G1期比例增加 ,并有明显的亚二倍体凋亡峰 ,细胞DNA抽提电泳后发现凋亡特征性梯状条带 ,端粒酶的活性显著受抑制。结论 :MG - 132能显著抑制食管癌细胞株Eca970 6的生长 ,并抑制端粒酶的活性 ,表明阻断泛素 -蛋白酶体通路可能是治疗食管癌的新途径
OBJECTIVE: To study the effects of blocking ubiquitin-proteasome pathway on esophageal cancer cell growth and telomerase activity. Methods: Different concentrations of MG - 132 were added to esophageal cancer cell line Eca9706 to specifically block the ubiquitin - proteasome pathway. The cell growth inhibition was measured by MTT assay. The changes of cell morphology, cell cycle and apoptosis were detected by flow cytometry (FCM). DNA fragment analysis further confirmed the existence of apoptosis and the activity of telomerase was detected. Results: MG - 132 could inhibit the growth of esophageal carcinoma cell line Eca9706 in a dose - and time - dependent manner. The cytopathic effect of MG - 132 was obvious under microscope. After acting on esophageal cancer cells, the proportion of G1 phase increased, and there was a significant sub-diploid apoptotic peak. The apoptosis characteristic ladder-like bands were found after DNA extraction and electrophoresis, and the activity of telomerase was significantly inhibited. CONCLUSION: MG - 132 can significantly inhibit the growth of esophageal cancer cell line Eca9706 and inhibit the activity of telomerase, indicating that blocking the ubiquitin - proteasome pathway may be a new way to treat esophageal cancer