目的:探讨不同年龄儿童胃泌素释放肽(GRP)水平及GRP与幽门螺旋杆菌(H. pylori)的相关性.方法:收集不同年龄儿童和确诊感染H. pylori儿童的血清和粪便,使用酶联免疫吸附检测法(ELISA)进行GRP水平的检测;通过口服或肛门感染建立NIH小鼠模型,探讨H. pylori感染对GRP分泌水平的影响.结果:不同年龄儿童血清中的GRP水平与年龄呈正相关,而粪便中的GRP水平与年龄呈负相关;H. pylori感染后,感染阳性组GRP水平显著高于感染阴性组;建立小鼠模型,与对照组相比口服和肛门组血“,”Objective:To investigate the level of gastrin-releasing peptide (GRP) in children of different ages and the correlation between GRP and Helicobacter pylori (H. pylori).Methods:Serum and feces of children of different ages and children with H. pylori infection were collected, and the level of GRP was detected by ELISA. The NIH mouse model was established by oral or anal infection, and the effect of H. pylori infection on GRP secretion level was discussed.Results:The serum GRP level of children of different ages was positively correlated with age, while the fecal GRP level was negatively correlated with age. After H. pylori infection, the GRP level in positive infection group was significantly higher than that in negative infection group. Compared with the control group, the secretion level of GRP in serum of oral and anal groups has no obvious change, but the secretion level of GRP in feces has increased significantly.Conclusion:The secretion level of GRP in children is related to age, and H. pylori infection can up-regulate the secretion level of GRP.