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目的:建立不同产地益智的HPLC指纹图谱,探讨不同产地化学成分的差异,为益智药材的质量鉴别与质量控制提供依据。方法:HPLC色谱法测定,采用国家药典委员会相似度评价软件进行分析。结果:不同产地益智指纹图谱有9个共有峰,不同产地益智指纹图谱的相似度均大于0.91。结论:不同产地益智化学成分相似,但成分的含量有差异。本方法稳定,重复性好,所建立的指纹图谱可用于益智药材的鉴别和质量控制。
OBJECTIVE: To establish the HPLC fingerprints of different producing areas of puzzle, to explore the difference of chemical composition in different producing areas, and to provide basis for the quality identification and quality control of the medicinal herbs. Methods: The HPLC chromatographic method was used to analyze the similarity of the National Pharmacopeial Committee. Results: There were 9 common peaks of fingerprint fingerprints in different areas, and the similarity of fingerprint fingerprints in different areas was greater than 0.91. Conclusion: The chemical composition of puzzle in different areas is similar, but the content of the ingredients is different. The method is stable and reproducible. The established fingerprint can be used for the identification and quality control of the medicinal herbs.