论文部分内容阅读
1986年10月～1987年6月我们对经胃镜检查的病人,无选择地取其胃窦部或幽门前区粘膜活检标本439份,作弯曲杆菌样细菌(CLO)的检测研究,现将结果报道如下。材料和方法439份活检标本分别作微生物学和组织学检查及部分电镜观察。1.涂片镜检:革兰氏染色,作油镜观察。2.分离培养及鉴定:将标本研磨后,接种于改良的Skirrow 选择培养基平皿上,置37℃的微需氧环境下培育,阳性者再作生物学特性鉴定。
From October 1986 to June 1987, we randomly selected 439 gastric mucosal biopsy specimens from the antrum or anterior pyloric region of patients undergoing gastroscopic examination for the detection of Campylobacter-like bacteria (CLO). The report is as follows. Materials and Methods 439 biopsy specimens were used for microbiological and histological examinations and partial electron microscopy observations. 1. Smear microscopy: Gram stain, for oil microscopy. 2. Isolation, culture and identification: The specimens were ground and inoculated on a modified Skirrow selection medium plate and incubated at 37°C in a microaerophilic environment. The positive subjects were then identified for biological characteristics.