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以特异性和敏感性较高的地高辛配基探针原位分子杂交及免疫组化技术，对云南６８例病毒性肝炎ＣＰＨ，ＣＡＨ，肝细胞癌及癌周组织进行ＨＢＶＤＮＡ，ＨＤＶＲＮＡ，ＨＢｓＡｇ，ＨＢｃＡｇ研究，结果表明：ＨＢｓＡｇ作为靶抗原诱发机体免疫反应，参与炎症过程；除浆膜型ＨＢｃＡｇ，弥漫型、膜型ＨＢｓＡｇ外，ＨＢＶＤＮＡ在肝组织中表达也与肝炎活动性有关；肝癌中ＨＢｓＡｇ与乙肝肝细胞无异，存在多种形式；在ＨＤＶＲＮＡ阳性病例中可存在ＨＢＶ复制，以ＨＤＶＲＮＡ原位分子杂交证实云南省为ＨＤＶ感染高发区，检出率１３２％．
In situ hybridization and immunohistochemistry of digoxigenin probe with high specificity and sensitivity were used to detect the expression of HBVDNA, HDVRNA, HBsAg in 68 cases of viral hepatitis CPH, CAH, hepatocellular carcinoma and peri-cancerous tissues , HBcAg study. The results showed that: HBsAg as a target antigen induced immune response, involved in the inflammatory process; in addition to serosal-type HBcAg, diffuse, membranous HBsAg, the expression of HBVDNA in liver tissue is also related to hepatitis activity; liver cancer HBsAg Which is similar to that of hepatitis B virus. There are many forms of HBV replication in HDV RNA positive cases. HDV RNA in situ hybridization confirmed that Yunnan Province is the high incidence area of HDV infection, with a detection rate of 13.2%.