论文部分内容阅读
Objective To investigate the mechanism of immune hyporesponsiveness induced by donor-antigen-unloaded recipient-derived immature dendritic cell (imDC) of liver grafts in rats.Methods Forty Sprague-Dawley rats (donor) and forty male Wistar rats (recipient) were randomly divided into 4 groups:control,cyclosporine A (CsA),mature DC (mDC),and imDC groups respectively,with 10 donor rats and 10 recipient rats in each group.Recipient rats in CsA group were treated with 10 mg·kg-1.d-1 CsA starting day 2 after the transplantation.Recipients in the mDC or imDC groups were given Wistar rat derived mDCs (1×106/rat) or imDCs (1×106/rat) via dorsal vein of the penis respectively 1 day before the transplantation.In each group,5 recipients were kept for determination of survival time and the other 5 rats were executed at day 10 after transplantation.Blood samples were collected for the measurement of serum alanine aminotransferase (ALT),total bilirubin (TBIL),interleulkin 2 (IL-2),interferon gamma (IFN-γ),IL-4,and IL-10 levels,liver tissue was harvested for HE staining and acute rejection evaluation.Expression levels of Fas-L/Fas in the grafts were detected by immunohistochemical staining; and Western blot was used to detect the expression level of Scurfin.Results The survival time of CsA and imDC groups was significantly longer than that of control and mDC groups (all P