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目的了解呼吸道感染患者肺炎衣原体感染状况,为临床诊断和治疗提供依据。方法 采集2006年1月在北京友谊医院发热门诊就诊的呼吸道患者的咽拭子样本88例,用肺炎衣原体种特异性引物对其进行PCR检测,阳性产物经基因测序,结果采用SPSS 10.0软件包进行数据统计分析,计数资料比较用χ2检验。结果 88例呼吸道感染患者的咽拭子样本中阳性者为28例,阳性率为31.82%。男性和女性阳性率差异无统计学意义。不同年龄组阳性率差异无统计学意义。结论 在有呼吸道症状的病例中,肺炎衣原体感染率较高,应对肺炎衣原体感染加以重视。PCR法是一简便、敏感和特异的诊断方法,值得在临床上进行应用和推广。
Objective To understand the status of Chlamydia pneumoniae infection in patients with respiratory tract infection and provide the basis for clinical diagnosis and treatment. Methods 88 cases of throat swabs were collected from respiratory patients in fever clinic of Beijing Friendship Hospital in January 2006. The PCR products were detected by PCR using primers specific for C. pneumoniae. The positive products were sequenced and the results were analyzed by SPSS 10.0 software package Data statistical analysis, count data comparison with χ2 test. Results 88 cases of respiratory infection in throat swab samples were positive in 28 cases, the positive rate was 31.82%. There was no significant difference in the positive rate between male and female. The positive rate of different age groups was no significant difference. Conclusions In cases of respiratory symptoms, the infection rate of Chlamydia pneumoniae is high, and Chlamydia pneumoniae infection should be paid attention to. PCR method is a simple, sensitive and specific diagnostic method, which is worthy of clinical application and promotion.