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目的:分别以玛瑙球和不锈钢球为球磨介质球磨法成功制备脱钙牙纳米材料,通过体外细胞毒性试验,对两种不同球磨介质制备的脱钙牙纳米材料的生物相容性进行了研究。方法:选用不同体积分数常温下的脱钙牙纳米材料浸提液为培养液,检测1d、3d、5d小鼠成纤维细胞L929的光吸收值,计算相对增殖率,确定脱钙牙纳米材料的毒性程度。结果:两种方法制备的脱钙牙纳米材料的细胞毒性为0级,有着良好的细胞生物相容性。以玛瑙为球磨介质制备的脱钙牙纳米材料的细胞相对增殖率大于同种条件下以不锈钢球为球磨介质制备的脱钙牙纳米材料的细胞相对增殖率。结论:以玛瑙为球磨介质制备的脱钙牙纳米材料较以不锈钢球为球磨介质制备的脱钙牙纳米材料具有较高的细胞相对增殖率。
OBJECTIVE: The successful preparation of decalcified dentin nanomaterials with agate ball and stainless steel ball as ball mill media respectively. The biocompatibility of decalcified dentin nanomaterials prepared by two different ball milling media was studied by in vitro cytotoxicity test. Methods: The decalcified dentin nano-material extracts with different volume fraction at room temperature were used as culture medium to measure the light absorption of L929 fibroblasts on day 1, day 3 and day 5, and the relative proliferation rate was calculated to determine the decalcified dentin nanomaterials Toxicity level. Results: The decalcification dentin nanomaterials prepared by the two methods have the cytotoxicity of grade 0 and have good cell biocompatibility. The relative cell proliferation rate of decalcified dental nanomaterials prepared with agate as the milling medium is greater than that of the decalcified dental nanomaterials prepared with stainless steel balls as the milling media under the same conditions. Conclusion: The decalcified dental nanomaterials prepared with agate as the milling media have higher relative cell proliferation rate than the decalcified dental nanomaterials prepared with stainless steel balls as the milling media.