Mixed strains Delftia sp.YH01 and Acidovorax sp.YH02, with capability of heterotrophic nitrification-aerobic de-nitrification, were introduced into a two-stage aerobic sequencing batch reactor to enhance NO-3-N removal. With optimal C/N of 8, efficient NO-3-N removal was achieved at initial NO-3-N concentration of 2000 mg·L-1. Meanwhile, the massive accumulation of NO-2-N was avoided during the long operation. Compared to the one-stage aerobic sequencing batch reactor, the removal efficiency of NO-3-N and TN in the two-stage aerobic se-quencing batch reactor was increased by 36.5％ and 42.7％, which respectively was 93.8％ and 88.4％. Microbial community study showed that the mixed strains have the stronger viability and can synergistically denitrify with the indigenous microorganisms in system, such as Azoarcus, Uncultured Saprospiraceae, Thauera, Paracocccus, which could be major contributors for aerobic denitrification. The proposed technology was shown to achieve high-efficiency treatment of high NO-3-N wastewater through aerobic denitrification.