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目的:探讨脑梗塞过程中大脑组织中的Rho激酶活性变化,揭示其潜在的临床价值。方法:大鼠左侧颈内动脉内注射月桂酸钠,诱导同侧脑半球发生脑梗塞,建立大鼠脑梗塞模型。在注射月桂酸钠前及注射后0.5h,3h及6h四个时间点上各处死大鼠6只,取双侧大脑实质组织,用于组织匀浆提取蛋白,通过ELISA法检测大鼠大脑实质组织中Rho激酶的活性。另取6h组大鼠的大脑组织用免疫荧光染色法检测Rho激酶底物-MBS的磷酸化情况。结果:在注射月桂酸钠之后3h及6h,同侧大脑实质(即梗塞侧)内的Rho激酶活性明显高于对侧正常脑实质内的Rho激酶活性(P<0.05),但在注射后0.5h内,双侧的Rho激酶活性无明显差异。结论:在大鼠脑梗塞模型中,大脑神经元内的Rho激酶活性能明显被活化,提示Rho激酶可能成为脑梗塞治疗的一个重要靶标。
Objective: To investigate the changes of Rho kinase activity in brain tissue during cerebral infarction and to reveal its potential clinical value. Methods: Rats in the left internal carotid artery injection of sodium laurate, induced cerebral infarction in the ipsilateral hemisphere, cerebral infarction model was established. Six rats were sacrificed at four time points before injection of sodium laurate and at 0.5h, 3h and 6h after injection. Bilateral parenchyma tissues were harvested for tissue homogenate extraction and protein content was detected by ELISA Rho kinase activity in tissues. Another 6h group of rats brain tissue immunofluorescence staining Rho kinase substrate-MBS phosphorylation. RESULTS: Rho kinase activity in ipsilateral brain parenchyma (ie, infarct side) was significantly higher than that in contralateral normal brain parenchyma (P <0.05) at 3h and 6h after injection of sodium laurate, but 0.5 h, bilateral Rho kinase activity no significant difference. CONCLUSION: Rho kinase activity in cerebral neurons can be obviously activated in rat cerebral infarction model, suggesting that Rho kinase may be an important target for the treatment of cerebral infarction.