目的探讨脑外伤患者脑脊液中兴奋性氨基酸的变化。方法用邻苯二甲醛柱前衍生后，采用梯度高效液相色谱荧光检测法测定４２例脑外伤，９例对照者脑脊液中兴奋性氨基酸———天门冬氨酸（Ａｓｐ）和谷氨酸（Ｇｌｕ）。结果４２例脑外伤患者脑脊液中Ａｓｐ，Ｇｌｕ测值（Ａｓｐ：１．８７８±０７２９μｍｏｌ／Ｌ，Ｇｌｕ：７．９８５±６．３２５μｍｏｌ／Ｌ）明显高于正常对照组（Ａｓｐ：１．３６２±０．２２０，Ｐ＜００５），Ｇｌｕ：１．９１４±１．５０７，Ｐ＜０００１）。２６例重伤组脑脊液中Ａｓｐ，Ｇｌｕ测值（２．４０２±１．１５７μｍｏｌ／Ｌ，１０．２５２±６．５７０μｍｏｌ／Ｌ）明显高于轻伤组（１．７３２±０．５９５μｍｏｌ／Ｌ，Ｐ＜０００１，４．３０１±３．７４１μｍｏｌ／Ｌ，Ｐ＜００５）。脑外伤预后良好（良好；中残）组（Ａｓｐ：１．８３５±０．７０５μｍｏｌ／Ｌ，Ｇｌｕ：６．３０７±４．１７１μｍｏｌ／Ｌ，ｎ＝２４）与预后不良（重残；植物生存；死亡）组（Ａｓｐ：２．５８８±１．０８９μｍｏｌ／Ｌ，Ｇｌｕ：１２．５３０±７．２６９μｍｏｌ／Ｌ，ｎ＝１８）相比较，二组差异有非常显著意义（Ａｓｐ：Ｐ＜００１，Ｇｌｕ：Ｐ＜０? Objective To investigate the changes of excitatory amino acids in cerebrospinal fluid of patients with traumatic brain injury. Methods 42 samples of traumatic brain injury and 9 controls were used to determine the excitatory amino acids - aspartate (Asp) and glutamate (GSH) in cerebrospinal fluid (CSF) after gradient derivatization with orthophthalaldehyde. Glu). Results Asp and Glu in cerebrospinal fluid (Asp: 1.878 ± 0.729μmol / L, Glu: 7.985 ± 6.325μmol / L) in 42 patients with traumatic brain injury were significantly higher than those in normal controls (Asp: 1.362 ± 0.220, P <005), Glu: 1.914 ± 1.507, P <0001). The values ​​of Asp and Glu in cerebrospinal fluid (2.402 ± 1.157μmol / L, 10.252 ± 6.570μmol / L) in 26 severely injured group were significantly higher than those in mild injury group (1.732 ± 0.595μmol / L, P <0001, 4.301 ± 3.741μmol / L, P <005). The patients with good prognosis of traumatic brain injury (Asp: 1.835 ± 0.705μmol / L, Glu: 6.307 ± 4.171μmol / L, n = 24) (Asp: 2.588 ± 1.089μmol / L, Glu: 12.530 ± 7.269μmol / L, n = 18), there were significant differences between the two groups , Glu: P <0?