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[目的]探讨PCNA和EGFR在大肠癌及癌旁粘膜细胞中的表达及意义。（方法）取正常大肠粘膜10例，大肠癌远分。近旁和癌粘膜吝叨例。应用免疫组化染色（SP法），在连续切片基础上同步检测PCNA和EGFR的表达。[结果]PCNA和EGFR阳性细胞在大肠癌组织中的分布呈异质性。正常大肠粘膜和大肠癌粘膜PCNA阳性细胞数分别为2．42±1．02和对20．21±6．58，两组间比较有极显著性差异（P＜0．01）；EGFR阳性率分别为0和73．33％，两组间比较也有显著性差异（P＜0．05）。大肠癌远分、近旁和癌粘膜PCNM阳性细胞数分别为小4.26±2．14、7．43±3.26和20.21±6．58，各组间比较均有极显著性差异（P＜0.01）；EGFR阳性率分别为3．33％、20％和73．33％，PCNA和EGFR的表达与大肠癌的分化程度、分期和淋巴结转移有关，与组织学类型无关。30例大肠癌粘膜PCNA和EGFR的表达呈显著性正相关（P＜0．05）。（结论）结合病理形态学观察，同时检测PCNA和EGFR的表达，能较客观准确地判断大肠癌的恶性程度和生物学特性，以及大肠癌切缘细胞的增殖活性和癌变潜能。
[Objective] To investigate the expression and significance of PCNA and EGFR in colorectal cancer and paraneoplastic mucosa cells. (Methods) 10 cases of normal colorectal mucosa and distant colorectal cancer were taken. Nearby and cancerous mucosa cases. Immunohistochemical staining (SP method) was used to simultaneously detect the expression of PCNA and EGFR on a continuous slice basis. [Results] The distribution of PCNA and EGFR positive cells in colorectal cancer tissues was heterogeneous. The number of PCNA positive cells in normal mucosa and colorectal mucosa was 2.42±1.02 and 20.21±6.58, respectively. There was a significant difference between the two groups (P<0.01); the positive rate of EGFR They were 0 and 73.33% respectively, there was also a significant difference between the two groups (P<0.05). The number of PCNM positive cells in distal, proximal and cancerous mucosa of colorectal cancer were 4.26±2.14, 7.43±3.26, and 20.21±6.58, respectively. There was a significant difference between the groups (P<0.01). The positive rates of EGFR were 3.33%, 20%, and 73.33%. The expressions of PCNA and EGFR were related to the differentiation degree, stage, and lymph node metastasis of colorectal cancer, but not related to the histological type. The expression of PCNA and EGFR in the mucosa of 30 patients with colorectal cancer was significantly positively correlated (P<0.05). (Conclusion) Combined with pathological observation, the simultaneous detection of PCNA and EGFR expression can objectively and accurately determine the degree of malignancy and biological characteristics of colorectal cancer, as well as the proliferative activity and cancer potential of colorectal cancer marginal cells.