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根据前列腺分泌蛋白 94(PSP94)和PSP5 7的基因结构特点 ,设计并合成了以地高辛标记的两条探针 ,利用组织原位杂交的方法 ,对增生和癌变前列腺组织中PSP94mRNA的表达量进行了比较。结果显示 ,2 0例增生前列腺组织 ,有 1 0例PSP94和PSP5 7共同探针的杂交信号比PSP94特异探针杂交信号强 ,1 0例相反。它们的共同点是均以腺体增生为主。而 7例前列腺癌组织中除 1例外 ,其余 6例PSP94特异探针的杂交信号均比PSP94和PSP5 7共同探针的杂交信号强。增生和癌变前列腺组织杂交结果比较 ,癌变前列腺组织PSP94特异探针杂交信号均比增生前列腺组织强。
According to the gene structure of prostatic secretory protein 94 (PSP94) and PSP5 7, two probes labeled with digoxigenin were designed and synthesized. The expression of PSP94 mRNA in prostatic hyperplasia and cancerous prostate tissues Compared. The results showed that in the 20 cases of prostatic hyperplasia, 10 hybridization signals of PSP94 and PSP5 7 probe were stronger than that of PSP94 probe, while the other 10 cases were opposite. Their common denominators are both gland hyperplasia. However, in all seven prostate cancer tissues, the hybridization signals of the other six PSP94-specific probes were stronger than that of PSP94 and PSP5-7 common probes. Hyperplasia and cancerous prostate tissue hybridization results, prostate cancer tissue prostate cancer PSP94 hybridization signals were stronger than the proliferation of prostate tissue.