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建立一种同时测定中药乙肝验方中芍药苷、党参炔苷、柴胡皂苷α、五味子乙素的HPLC方法。样品经50%乙醇、60℃水浴加热结合微波辅助法提取,选用Hypersil C18柱(5μm,250mm×4.6mm i.d),以乙腈-1%冰醋酸水溶液进行梯度洗脱,流速1.0ml/min,柱温25℃,检测波长280nm。结果显示4种指标成分在各自线性范围内相关系数均在0.999以上,平均加标回收率在96.3%~98.5%之间,RSD在1.5%~2.7%之间。样品测定结果分别为42.41mg/g、0.36mg/g、12.18mg/g、2.97mg/g。检测方法灵敏、简便、快速、准确,适用于中药验方药效成分的质量控制和定量分析。
To establish a HPLC method for simultaneous determination of paeoniflorin, Codonopin, Saikosaponin a and Schisandrin B in traditional Chinese medicine Hepatitis B prescription. The samples were extracted with 50% ethanol and 60 ℃ water bath combined with microwave-assisted extraction. The samples were separated on a Hypersil C18 column (5μm, 250mm × 4.6mm id) with a gradient of acetonitrile-1% glacial acetic acid at a flow rate of 1.0ml / Temperature 25 ℃, detection wavelength 280nm. The results showed that the correlation coefficients of the four index components were all above 0.999 in the linear range, with the average recoveries between 96.3% and 98.5% and RSD between 1.5% and 2.7%. The measurement results of the samples were 42.41 mg / g, 0.36 mg / g, 12.18 mg / g and 2.97 mg / g, respectively. The detection method is sensitive, simple, rapid and accurate, suitable for the quality control and quantitative analysis of the prescription of traditional Chinese medicine.