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为明确北京地区茄子感染番茄褪绿病毒(Tomato chlorosis virus,To CV)的情况,于2015年3—6月收集了10份疑似感染To CV的设施茄子样品,通过PCR分子鉴定法进行检测,并进一步对扩增阳性样品To CV的CP基因采用邻接法进行了系统进化分析。结果表明,10个检测样品中有4个扩增得到约463 bp的特异条带,经测序与Gen Bank中To CV序列相似性达到99.0%以上,表明这4个样品均携带有该病毒,检出率为40%;To CV茄子分离物的CP基因序列大小为774 bp,Gen Bank登录号为KT751008,与To CV北京番茄分离物(KC311375)同源性最高,达到99.9%,确认该序列为To CV片段;系统进化树显示,To CV茄子分离物与To CV日本分离物(AB513443)处于同一分支,具有密切的亲缘关系,表明不同国家To CV分离物之间的亲缘关系与地理距离具有一定的相关性。
To clarify Tomato chlorosis virus (ToCV) infection in Beijing, 10 samples of eggplant were collected from To CV in March-June 2015. The PCR products were analyzed by PCR. Further analysis of the phylogenetic tree based on the contiguous method was carried out on the CP gene that amplified the positive sample To CV. The results showed that 4 out of 10 samples amplified specific bands of about 463 bp. The sequence similarity to To CV in Gen Bank was above 99.0%, indicating that the 4 samples carried the virus The percentage of CP gene in To CV was 774 bp, GenBank accession number KT751008, which had the highest homology (99.9%) with To CV Beijing tomato isolate (KC311375), and the sequence was found to be To CV fragments. Phylogenetic tree showed that To CV isolates of To CV and isolates of To CV Japan (AB513443) were in the same branch and had a close genetic relationship, indicating that the genetic relationship between To ToV isolates in different countries and geographical distance was certain Relevance.