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通过对从甘肃的张掖、武威、天祝、秦王川、甘南等地的大麦和青稞叶片及种子上共分离得到的11个条纹病病菌菌株进行致病力测定。结果显示,不同菌株在致病力等方面存在明显差异。其中菌株TZ1和LQ1致病力最强,离体叶片接种和拌种接种在大麦叶片上发病率均达到80%以上,显著高于其他菌株。将这11个菌株基因组DNA经RAPD分析表明,不同菌株间的扩增图谱差别明显,表现出各菌株间明显的遗传多样性。经聚类分析显示,大麦条纹病病菌不同菌株的RAPD类群与致病力和不同寄主有一定的相关性。
Pathogenicity of 11 strains of pathogenic bacteria were isolated from leaves and seeds of barley and barley from Zhangye, Wuwei, Tianzhu, Qinwangchuan, Gannan, Gansu and other places. The results showed that different strains in pathogenicity there are significant differences. The strains TZ1 and LQ1 had the strongest virulence. The rates of inoculation and seed dressing inoculation on leaves of barley were more than 80%, significantly higher than other strains. The RAPD analysis of the genomic DNA of these 11 strains showed that the amplified strains differed significantly among different strains and showed obvious genetic diversity among the strains. The clustering analysis showed that the RAPD groups of different strains of pathogen of barley were associated with pathogenicity and different hosts.