目的　建立容易检测的丙型肝炎病毒 (HCV)细胞模型。方法　利用基因重组技术 ,构建HCVcDNA与荧光素酶 (luc)融合基因可调控逆转录病毒载体 ;通过脂质体介导方法转染人肝癌细胞 (HHCC) ,观察luc基因的表达。结果　①成功地将luc基因融合于HCVC区和大部分E1区基因的下游 ,构建了带HCV luc融合基因的真核表达载体 (pBPST HCV luc) ;②该载体在细胞内有效表达luc活性 ,puromycin筛选可提高luc的表达水平 ,并可受四环素的调节。结论　初步建立了表达HCVC E1和luc融合基因的可调控转染细胞模型 ,为以HCVC E1为靶的基因治疗提供研究工具 Objective To establish an easily detectable hepatitis C virus (HCV) cell model. Methods Recombinant retroviral vector containing HCV cDNA and luciferase (luc) fusion gene was constructed by gene recombination technique. The expression of luc gene was detected by liposome mediated transfection of human hepatoma cells (HHCC). RESULTS: The luc gene was successfully fused to the downstream of the HCVC region and most of the E1 region genes to construct the eukaryotic expression vector (pBPST HCV luc) with the HCV luc fusion gene. The vector effectively expressed luc activity in cells and puromycin Screening can increase luc expression levels and can be regulated by tetracycline. Conclusion A transfected cell model expressing the fusion gene of E1 and luc of HCVC was initially established, providing a research tool for gene therapy targeting HCVC E1