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[Objective] This study aimed to investigate the mechanism of apoptosis induced by ligustrazine(TMP) and cis-dichlorodiamine platinum(DDP) in SGC-7901 cell lines in vitro. [Methods] SGC-7901 cell lines were treated with ligustrazine and DDP alone or combined for 48 h for Western blot analysis, respectively. Western blot analysis was used to determine the expression of proteins involved in apoptosis including NF-κB p65, bax and caspase-3. [Results] The viability of SGC-7901 cells was inhibited after treated with ligustrazine and/or combined with DDP. The expression of NF-κB P65 protein decreased after treated with drugs, in which the protein decreased significantly in 1.2 mg/ml of TMP combined with 2 μg/ml of DDP group.Meanwhile, we investigated the protein expression of bax and caspase-3. The results showed that the expression of the two proteins increased following with the increasing concentration of TMP. [Conclusion] All the results indicated that ligustrazine combined with DDP could induce the apoptosis of SGC-7901 cell lines, and NF-κB maybe the possible way to induce the cell apoptosis.
[Objective] This study aimed to investigate the mechanism of apoptosis induced by ligustrazine (TMP) and cis-dichlorodiamine platinum (DDP) in SGC-7901 cell lines in vitro. [Methods] SGC-7901 cell lines were treated with ligustrazine and DDP alone or combined for 48 h for Western blot analysis, respectively. Western blot analysis was used to determine the expression of proteins involved in apoptosis including NF-κB p65, bax and caspase-3. [Results] The viability of SGC-7901 cells was inhibited after treated with ligustrazine and / or combined with DDP. The expression of NF-κB P65 protein decreased after treated with drugs, in which the protein decreased significantly in 1.2 mg / ml of TMP combined with 2 μg / ml of DDP group. we investigated the protein expression of bax and caspase-3. The results showed that the expression of the two proteins increased with the increasing concentration of TMP. [Conclusion] All the results indicated that ligustrazine combined with DDP co uld induce the apoptosis of SGC-7901 cell lines, and NF-κB maybe the possible way to induce the cell apoptosis.