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目的探索微载体培养细胞大量制备甲肝病毒抗原及其灭活疫苗的可行性。方法使用 Cytodex- 1培养恒河猴胚肾细胞和 Vero细胞制备 HAV ,经过初步纯化、甲醛灭活、吸附佐剂 ,制成甲肝灭活疫苗 ,免疫昆明种小白鼠 ,测定免疫原性。结果 HAV X株和 W株抗原滴度分别为 1∶ 2 5 6、1∶ 12 8,感染滴度 (log TCID5 0 / m l)分别为 8.5 0、8.17,与静止培养获得的滴度相当。小鼠抗 HAV抗体第 45 d达到峰值 ,滴度分别为 1∶ (96 .0± 78.4)、1∶ (12 8.0± 70 .1)。结论实验性甲肝灭活疫苗具有良好的免疫原性 ,应用微载体培养细胞制备甲肝灭活疫苗是可行的。
Objective To explore the feasibility of large-scale preparation of hepatitis A virus antigen and its inactivated vaccine by microcarrier cultured cells. Methods HAV cells were cultured with Cytodex-1 and cultured in Vero cells. After primary purification, formaldehyde inactivation and adjuvant adsorption, a hepatitis A inactivated vaccine was prepared and immunized Kunming mice. Immunogenicity was determined. Results The titer of HAV X strain and W strain antigen were 1:25 56 and 1:12 8, respectively, and the log TCID 50 / ml was 8.5 0 and 8.17, respectively, which was comparable to the titer obtained in static culture. The anti-HAV antibodies of mice reached the peak on the 45th day with the titers of 1: (96.0 ± 78.4) and 1: (12 8.0 ± 70.1), respectively. Conclusion The experimental hepatitis A inactivated vaccine has good immunogenicity, and it is feasible to use the microcarrier cultured cells to produce hepatitis A inactivated vaccine.