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目的:探讨佛甲草提取液(SLT)对实验性肝纤维化大鼠脂质过氧化的影响。方法:雄性SD大鼠随机分为正常对照组、模型组、阳性对照组(Col,0.1 mg·kg-1)、SLT低剂量组(SLT,4 g·kg-1)、SLT高剂量组(SLT,8 g·kg-1)。采用50%的CCl4花生油溶液灌服制备大鼠肝纤维化模型,期间给予灌服SLT(4,8 g·kg-1)、Col(0.1 mg·kg-1)进行干预,共9周。9周后处死大鼠取固定部位肝组织及血清标本,光镜下观察肝细胞结构和肝纤维化程度,分光光度法检测肝组织匀浆、血清中丙二醛(MDA)含量以及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性的变化。结果:光镜下可见模型组大鼠肝细胞严重变性、坏死,胶原纤维明显增加;肝组织、血清中MDA含量增高,SOD、GSH-Px活力显著下降。SLT预防给药明显改善大鼠肝细胞结构,减轻肝纤维化程度;明显降低肝纤维化大鼠肝脏及血清MDA含量,显著升高SOD、GSH-Px活性。结论:佛甲草提取液对大鼠肝纤维化有一定的防治作用,其机制可能与抗脂质过氧化损伤有关。
Objective: To investigate the effects of extract of Achilles tendonum (SL) on lipid peroxidation in experimental hepatic fibrosis rats. Methods: Male SD rats were randomly divided into normal control group, model group, positive control group (Col, 0.1 mg · kg -1), low dose SLT group (4 mg · kg -1) and SLT high dose group SLT, 8 g · kg-1). The models of hepatic fibrosis were established by intragastric administration of 50% CCl4 peanut oil solution. Serum SL (4, 8 g · kg-1) and Col (0.1 mg · kg-1) were administrated for 9 weeks. After 9 weeks, the rats were sacrificed to take the fixed part of the liver tissue and serum samples. The liver cell structure and hepatic fibrosis were observed under the light microscope. The content of malondialdehyde (MDA) and the content of malondialdehyde (MDA) in serum were detected by spectrophotometry. The changes of activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). Results: Under the light microscope, the hepatocytes in the model group were severely degenerated, necrotic and collagen fibers were significantly increased. The content of MDA in liver tissue and serum was increased, and the activities of SOD and GSH-Px were significantly decreased. The administration of SLT could obviously improve the structure of rat hepatocytes, reduce the degree of hepatic fibrosis, significantly reduce the content of MDA in liver and serum of rats with hepatic fibrosis, and significantly increase the activity of SOD and GSH-Px. CONCLUSION: The extract of Achilles tendon has some preventive and therapeutic effects on hepatic fibrosis in rats, which may be related to the anti-lipid peroxidation injury.