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目的:探讨子宫颈鳞癌组织中p21和VEGF蛋白的表达及其与临床病理特征的关系。方法:采用免疫组织化学技术,检测56例子宫颈鳞癌、24例宫颈原位癌和16例癌旁正常宫颈上皮中p21和VEGF的表达,分析其表达与宫颈鳞癌的临床分期、病理类型及有无淋巴结转移的关系。结果:p21在宫颈鳞癌、原位癌和癌旁正常宫颈上皮中的阳性表达率分别为51.79%、83.33%、93.75%;VEGF为82.14%、58.33%、31.25%。p21和VEGF在癌旁正常宫颈上皮组与鳞癌组及原位癌组相比较差异均有统计学意义(P<0.01)。p21和VEGF的阳性表达率与临床分期、病理类型及有无淋巴结转移均有明显相关性(P<0.01);p21和VEGF在宫颈鳞癌的表达中呈负相关(P<0.01)。结论:宫颈鳞癌组织中p21蛋白表达水平的降低和VEGF蛋白表达水平的上调,可能参与宫颈鳞癌的浸润和转移,两者的联合监测对宫颈鳞癌的诊断、临床分期、预后有指导作用。
Objective: To investigate the expression of p21 and VEGF in cervical squamous cell carcinoma and its relationship with clinicopathological features. Methods: Immunohistochemical technique was used to detect the expression of p21 and VEGF in 56 cases of cervical squamous cell carcinoma, 24 cases of cervical carcinoma in situ and 16 cases of adjacent normal cervical epithelium. The expression of p21 and VEGF in cervical squamous cell carcinoma was analyzed by immunohistochemistry. With or without lymph node metastasis. Results: The positive rates of p21 expression in cervical squamous cell carcinoma, carcinoma in situ and adjacent normal cervical epithelium were 51.79%, 83.33% and 93.75% respectively; VEGF was 82.14%, 58.33% and 31.25% respectively. There was significant difference between p21 and VEGF in adjacent normal cervical epithelium and squamous cell carcinoma and carcinoma in situ (P <0.01). The positive rates of p21 and VEGF were significantly correlated with the clinical stage, pathological type and lymph node metastasis (P <0.01). There was a negative correlation between the expressions of p21 and VEGF in cervical squamous cell carcinoma (P <0.01). Conclusion: The decrease of p21 protein and the upregulation of VEGF protein in cervical squamous cell carcinoma may be involved in the infiltration and metastasis of cervical squamous cell carcinoma. The combined monitoring of cervical intraepithelial neoplasia and cervical carcinoma may play a guiding role in the diagnosis, clinical stage and prognosis of cervical squamous cell carcinoma .