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本研究以掘氏疫霉Phytophthora drechsleri Tucker野生型菌株的单游动孢子无性系为亲本,测定了自交、杂交后代交配型的遗传,经KMnO_4处理引起的交配型变异以及F_1代可自孕单卵孢株有性生殖后代交配型的遗传与变异。聚碳膜间隔配对诱导A_1和A_2菌株自交产生的卵孢子经H_2O_2处理刺激萌发(萌发率12～16％)获得单卵孢株。交配型测定结果表明,A_1和A_2亲本自交S_1代单卵孢株均保持与亲本一致的交配型。用KMnO_4处理上述卵孢子导致A_1和A_2亲本的部分S_1代单卵孢株出现自孕现象,少数A_1亲本自交后代改变为A_2交配型;从A_1交配型亲本的S_1代可自孕菌株产生的卵孢子萌发所建立的单卵孢株中,同时获得A_1和A_2交配型菌株。上述结果不支持Sansome关于疫霉菌A_1、A_2交配型分别由纯合、杂合基因控制的假说,进一步证明了Ko关于交配型抑制因子控制交配型表达的假说的合理性。掘氏疫霉种内菌株直接配对产生的卵孢子用H_O_2刺激萌发获得F_1代单卵孢株。测定结果表明,在F_1代出现A_1、A_2、A_1A_2、A~04种交配型的单卵孢株,其比例因不同亲本组合而有较大差异。 F_1代出现的A_1A_2菌株自孕产生的卵孢子经H_2O_2处理刺激萌发建立自交系,观察到A_1A_2交配型在有性生殖后代发生分离,各交配型比例因亲本不同而异。A_1A_2菌株的自孕能力在单游动孢子无性系后代可稳定遗传,认为F_1代出现的A_1A_2个体来自亲本的杂交。A_1A_2单卵孢株保藏4～6个月后大多仍具自孕能力,少数改变为A_2交配型。
In this study, a single zoospore clone of the Phytophthora drechsleri Tucker wild type strain was used as a parent to determine the inheritance of the mating type in the selfing and crossing progenies, the mating type variation caused by KMnO_4 treatment, Inheritance and Variation of Mating Type of Ovospore Strains with Sexual Reproduction. Polysorbate-paired-paired induction of A_1 and A_2 strains of oospores produced by self-fertilization by H_2O_2 treatment to stimulate germination (germination rate of 12 to 16%) to obtain a single oospores. The results of mating type assay showed that the mating type of the self-crossed S_1 mono-oospores of A_1 and A_2 all maintained the same mating pattern as the parents. Treatment of these oospores with KMnO_4 resulted in some S_1 mono-sporozoites in A_1 and A_2 parents, and a few A_1 parents were selfed to A_2 mating type. The S_1 generation from mating type A_1 maternal inbred strains Oospore germination established by single spore strains, at the same time get A 1 and A 2 mating strains. The above results do not support the hypothesis that Sansome’s control of the mating type of hybrids A_1 and A_2 by homozygous and heterozygous genes, respectively, further confirms the rationality of Ko’s hypothesis that mating suppressors control mating type expression. Phytophthora sp. Strains produced by direct inoculation of Phytophthora sojae strains were stimulated with H_O_2 to obtain F_1 mono-oospores. The results showed that there were great differences in the proportion of single mating strains of A_1, A_2, A_1A_2 and A ~ 04 in F_1 generation due to different combinations of parents. The self-conceived oospores produced by F_1 generation of A_1A_2 strain were stimulated by H_2O_2 to establish inbred lines. It was observed that the mating type of A_1A_2 was segregated in the offspring of sexual reproduction. The proportion of each mating type varies with the parents. The self-pregnant ability of A_1A_2 strain can be inherited stably in the offspring of the single zoospore clones, and the A_1A_2 individuals in the F_1 generation came from the cross of the parents. After being preserved for 4 to 6 months, most of A_1A_2 oospores were still capable of self-fertilization, and a few changed to A_2 mating type.