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目的:研究中药黄芪注射剂预处理对大鼠脊髓缺血再灌注模型神经细胞凋亡表达的影响,探讨中药黄芪注射液对大鼠脊髓缺血再灌注模型神经细胞的作用效应及其作用机理。方法:采用大鼠肾动脉分支点下阻断腹主动脉的方法,建立大鼠脊髓缺血再灌注模型。90只SD大鼠采用单纯随机抽样方法分为假手术组(A组,n=30)、缺血再灌注组(B组,n=30)、黄芪注射液预处理组(C组,n=30)。其中假手术组开腹后只暴露腹主动脉不阻断腹主动脉,然后关闭腹腔;缺血再灌注组阻断腹主动脉30min后,放开腹主动脉进行缺血再灌注;黄芪注射液预处理组开腹前,经大鼠尾静脉注射黄芪注射液(10mL/kg),然后开腹阻断腹主动脉30min后,放开腹主动脉进行缺血再灌注。造模完成后,各组动物分别于缺血再灌注12h、1d、2d、3d、7d后用Tarlor法评价大鼠双后肢神经功能,然后取出L2-4段脊髓进行苏木精-伊红染色观察脊髓组织病理学变化,并用原位末端标记法(TUNEL)检测凋亡细胞,计算凋亡指数。结果:黄芪注射液预处理可以减轻大鼠缺血再灌注模型双后肢神经功能损伤情况(P<0.05)、可以减少脊髓缺血再灌注后脊髓神经细胞中凋亡细胞数量(P<0.05)。
Objective: To study the effect of astragalus injection on nerve cell apoptosis in spinal cord ischemia / reperfusion model in rats and the effect and mechanism of Radix Astragali injection on neurons in spinal cord ischemia-reperfusion model in rats. Methods: A rat model of spinal cord ischemia-reperfusion was established by blocking the abdominal aorta with the branch of renal artery. Ninety Sprague-Dawley rats were randomly divided into three groups: sham operation group (n = 30), ischemia reperfusion group (n = 30), Astragalus injection pretreatment group (n = 30). The sham-operation group only exposed the abdominal aorta without blocking the abdominal aorta and then closed the abdominal cavity. The ischemia-reperfusion group blocked the abdominal aorta for 30 minutes and then the abdominal aorta was opened for ischemia-reperfusion. Astragalus injection The rats in the pretreatment group were injected with Astragalus injection (10 mL / kg) through the tail vein of the rats before they were given laparotomy. Then the abdominal aorta was opened by laparotomy for 30 min and the abdominal aorta was released for ischemia-reperfusion. After the model was established, the neurological function of the double hindlimb in rats was assessed by Tarlor method at 12h, 1d, 2d, 3d, 7d after ischemia reperfusion, and then the L2-4 spinal cord was removed for hematoxylin-eosin staining Pathological changes of spinal cord were observed. Apoptotic cells were detected by TUNEL and apoptosis index was calculated. Results: Astragalus membranaceus injection pretreatment can reduce the neurological impairment of both hindlimbs (P <0.05), reduce the number of apoptotic cells in the spinal cord neurons after spinal cord ischemia-reperfusion (P <0.05).