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为解决利用微生物法处理酸性矿山废水中菌种扩大培养、菌种保存和接种等问题,研究利用固态发酵法制备硫酸盐还原菌(SRB)固定化产品。实验以亚硫酸盐还原酶(SiR)作为菌种活性检测指标,进行生物质粒径、初始含水率、接种量单因素实验,响应面法确定各生物质载体固态发酵的最优条件并验证;用固载产品处理模拟酸性矿山废水数据按照Lineweaver-Burk作图法,建立SRB菌去除SO2-4的反应动力学方程。结果表明,以玉米芯为载体最佳发酵条件为:粒径8~20目、含水率70%、接种量10%,优化后SiR酶活为2.7385 U/g;木屑:粒径1~8目、含水率80%、接种5%,优化后SiR酶活为2.4863 U/g;甘蔗渣:8~20目、含水率75%、接种10%,优化后SiR酶活为4.2960 U/g。固载产品处理模拟酸性矿井水SO2-4,以1/v对1/[s]作图,玉米芯固载SRB菌的图相关性最好y=1176.9x+0.6034,R2=0.9199,建立玉米芯固载SRB菌的处理SO2-4动力学方程为V=1.657[s]/(1950.45+[s])。
In order to solve the problems of expanding culture, strain preservation and inoculation in the acid mine wastewater by using the microorganism method, the solid state fermentation method was used to prepare the sulfate-reducing bacteria (SRB) immobilized product. Sulfite reductase (SiR) was used as an indicator to detect the activity of bacteria, and the biomass particle size, initial moisture content and inoculum size were tested by single factor experiment. The optimum conditions for solid state fermentation of biomass carriers were determined by response surface methodology. Treatment of Simulated Acid Mine Wastewater with Immobilized Products According to the Lineweaver-Burk plotting method, the kinetic equation of SRB bacteria removal SO2-4 was established. The results showed that the optimal fermentation conditions for corncob as carrier were as follows: particle size 8-20 mesh, water content 70%, inoculation 10%, optimized SiR activity 2.7385 U / g; wood chips 1-8 mesh , Water content 80%, inoculation 5%, optimized SiR activity of 2.4863 U / g; bagasse: 8-20 mesh, water content 75%, inoculated 10%, optimized SiR activity of 4.2960 U / g. The solid product was processed to simulate acidic mine water SO2-4 plotted as 1 / v versus 1 / [s] with the best correlation between corncob immobilized SRB bacteria and y = 1176.9x + 0.6034 and R2 = 0.9199 to establish corn The SO2-4 kinetics equation for the SRB-loaded SRB bacteria was V = 1.657 [s] / (1950.45+ [s]).