心肌组织于-20℃恒冷切片机中连续切片,贴于载玻片上。~(125)I-心得静(30～300pM,本室标记)滴加于玻片组织上,在37℃、pH7.4缓冲液下孵育,含或不含L-心得舒10~(-4)M,4℃缓冲液震荡洗脱2次。平行做二组,一组刮下直接测定cpm数;一组留做自显影分析。浸膜法将核4乳胶涂布于载玻片上,避光晾干。与组织片按绞链法密贴,曝光、显影、定影。用Ommicon 3600(USA)计算机图像信息分析仪进行银粒计数。蛋白 Myocardial tissue was serially sectioned in a -20 ° C cryostat and attached to a glass slide. ~ (125) I-Qujing static (30 ~ 300pM, room mark) was added dropwise on glass tissue, incubated at 37 ℃, pH7.4 buffer, with or without L- ) M, 2 times shaking at 4 ℃ buffer. Parallel to do two groups, a group scrape directly determine the number of cpm; a group to do self-imaging analysis. Immersion method The core 4 latex coated on a glass slide, dry away from light. Tissue film and hinge press secret law, exposure, development, fixing. Silver counts were made using an Ommicon 3600 (USA) computer image analyzer. protein