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CBF基因不仅是植物CBF抗冷途径的枢纽,而且可以提高植物的抗冷能力.而植物的抗冷能力的提高是由于CBF基因调控下游抗冷基因的表达来实现的.本研究根据前期己克隆得到的绵果荠LlCBF基因(JQ687132)序列,利用TAIL-PCR方法从新疆早春短命植物绵果荠基因组DNA中分离得到LlCBF基因编码区上游1 172 bp启动子序列;将该序列连入克隆载体并测序,通过生物信息学分析表明,该序列含TATA-box、CAAT-box等典型的真核生物核心启动子元件,同时具有等逆境响应相关元件.在烟草叶片中的瞬时表达分析,进一步表明,在低温、干旱及盐胁迫诱导下该启动子序列具有驱动GUS报告基因表达的特性.本研究对新疆早春短命植物种质资源的开发利用及丰富作物逆境基因工程诱导启动子资源具有重要的实践意义.“,”CBF gene plays important roles in survival of plants under cold stress by serving as master regulator of sets of downstream cold-responsive genes.In present study,based on LlCBF gene (JQ687132) sequence that cloned in prophase research,a 1 172 bp promoter sequence of 5'upstream of initial codon of LlCBF gene from early spring ephemeral Lachnoloma lehmannii was cloned with TAIL-PCR method.The promoter sequence was analyzed bioinformatically in PlantCARE cisacting element database.The results showed that this promoter contains many typical eukaryotic core promoter elements,such as TATA-box and CAAT-box,and many cis-acting elements involved in the stress responses in plant,such as LTR,ABRE,MBS,CE3 and TC-rich repeats.Moreover,the results of transient expression assays in tobacco leaves showed that this promoter could drive the expression of GUS reporter gene under induction of cold,salt and drought stress.This study is important for the utilization of early spring ephemeral plant and the development of the inducible promoter in genetic engineering of crop.