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摘要目的验证在用短T1对比剂在体内选择性标记单核细胞后的MR成像R1(R1=1/T1)映射,是否能在衡量梗死愈合程度时在时间与空间上得出动力学定量检测结果。材料与方法所有的实验步骤将使用小鼠进行,研究已经获得了动物研究机构委员会的批准。100μL的双标记脂质(DLL)包括钆(Gd)-二乙三胺五乙酸(DTPA)二(硬脂酰胺)和Dil染料用于在心肌梗死(MI)发生2天前标记单核细胞。通过闭塞左前降支冠状动脉1h然后再灌注的方法制作MI模型。
Abstract Objective To verify MR imaging R1 (R1 = 1 / T1) mapping after selective labeling of monocytes with a short T1 contrast agent in vivo to determine kinetic quantitative results in time and space when measuring infarct healing . Materials and Methods All experimental procedures will be performed using mice, and the study has been approved by the Animal Research Institutions Board. 100 μL of dual-labeled lipids (DLLs) included gadolinium (Gd) -diethylenetriaminepentaacetic acid (DTPA) bis (stearamide) and Dil dyes for labeling of monocytes 2 days prior to myocardial infarction (MI). The MI model was made by occluding the left anterior descending coronary artery for 1 h followed by reperfusion.