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作者用ABC免疫组化方法观察了鼠抗人胃癌单克降抗体MG 7.MG 9,MGd1相应抗原在不同胎龄胎儿(10～40周)多种组织中的表达及其消长过程。胎儿组织用10％缓冲福尔马林或1.33mol/L多聚甲醛或0.0087 mol/L苦味酸混合固定液固定,石蜡包埋,部分胎儿组织同时作冰冻切片染色。结果发现:MG7,MG9,MGd1均与14～36周胎儿食管上皮表层细胞、15～32周胎儿结肠上皮表面上皮细胞及部分胎胃上皮呈不同程度的阳性反应。抗原表达随胎龄变化:第14周见于食管上皮,第15周见于结肠上皮,表达较弱,第20～24周表达最强,第28周以后表达逐渐减弱,足月胎儿抗原表达完全消失。这组抗体相应抗原在任何时期的胎儿肝、胆囊、胰腺、小肠、心肌、肺、气管、肾、肾上腺、骨骼肌、皮肤等组织均无表达,仅与2例接近成熟的胎儿胸腺小体呈强阳性反应。由于本组抗体相应抗原仅在胃癌、食管癌、结肠癌及其相应胎儿组织中表达,而在正常人胃、食管、结肠等组织表达阴性,推测它们是一组与胃癌相关的消化道癌胚共同抗原。
The authors used ABC immunohistochemical method to observe the expression and lengthening process of the corresponding antigen of mouse anti-human gastric cancer monoclonal antibody MG 7.MG 9, MGd1 in different fetal ages (10-40 weeks). Fetal tissues were fixed with 10% buffered formalin or 1.33 mol/L paraformaldehyde or 0.0087 mol/L picric acid mixed with fixative, embedded in paraffin, and some of the fetal tissues were stained with frozen sections at the same time. The results showed that MG7, MG9, and MGd1 all showed positive responses to esophageal epithelial surface cells at week 14 to 36, fetal epithelial cells at fetal epithelium at weeks 15 to 32, and some fetal epithelium. Antigen expression varies with gestational age: 14th week was seen in the esophageal epithelium, and 15th week in the colonic epithelium. It was weakly expressed, and it was the strongest in the 20th to 24th week. The expression gradually weakened after the 28th week and the full-term fetal antigen expression completely disappeared. This group of antibody-associated antigens showed no expression in fetal liver, gallbladder, pancreas, small intestine, myocardium, lung, trachea, kidney, adrenal gland, skeletal muscle, and skin at any time. It was only present in 2 cases of mature fetal thymus bodies. Strong positive reaction. Since this group of antibody-associated antigens is only expressed in gastric cancer, esophageal cancer, colon cancer, and their corresponding fetal tissues, but in normal human stomach, esophagus, colon, and other tissues, it is assumed that they are a group of gastric cancer-related gastrointestinal cancer embryos. Common antigen.